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A second-step splicing activity is conserved from yeast to human

AutorCicarelli, R. M. B.; Khaouja, Amina; Codony, Carles; Espuny, Ruth; Eritja Casadellà, Ramón; Bach-Elias, Montse
Fecha de publicación18-jun-1998
EditorElsevier
CitaciónBiochemical and Biophysical Research Communications 247(2): 204-206 (1998)
ResumenWe describe a defective HeLa nuclear extract which is particularly deficient in step 2 of splicing reaction. With this extract we have studied the conservation of a second-step activity from yeast to human cells. We detected a S. cerevisiae second-step splicing activity that allows restoration of step 2 of the defective HeLa nuclear extract, which indicates that yeast purified fraction has a second-step activity that is conserved from yeast to human cells. The activity is a yeast UsnRNP protein(s) since it is purified with anti-tri-methylguanosine by immunoaffinity columns.
Versión del editorhttp://dx.doi.org/10.1006/bbrc.1998.8771
URIhttp://hdl.handle.net/10261/109168
DOI10.1006/bbrc.1998.8771
Identificadoresdoi: 10.1006/bbrc.1998.8771
issn: 0006-291X
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