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Characterization of phenolic glucosides from soybean root nodules by ion-exchange high performance liquid chromatography, ultraviolet spectroscopy and electrospray mass spectrometry

AuthorsMorán, José F. ; Klucas, R. V.; Grayer, Renée J.; Abián, Joaquín ; Harborne, Jeffrey B.; Becana Ausejo, Manuel
KeywordsGlycine max
soybean nodules
nitrogen fixation
phenolic acid glucosides
electrospray mass spectrometry
high performance liquid chromatography
ultraviolet spectroscopy
Issue DateJul-1998
PublisherJohn Wiley & Sons
CitationPhytochemical Analysis 9 (4): 171–176 (1998)
Abstracton-exchange high performance liquid chromatography (HPLC) with an Aminex column and photodiode-array detection was used to purify, from soybean root nodules, highly polar phenolic acid conjugates that are recalcitrant to isolation by conventional techniques based on reversed-phase chemistry. HPLC, acid and enzymatic hydrolysis, ultraviolet spectroscopy, high resolution fast atom bombardment mass spectrometry (MS) and single and tandem electrospray MS were used to identify the 4-O-β-glucosides of p-hydroxybenzoic, protocatechuic and vanillic acids (which are reported in legumes for the first time) and the 5-O-β-glucoside of gentisic acid. The four phenolic acids, as well as gallic acid and its methyl ester, were also found in soybean nodules.
Description6 Pags.- 3 Tabls.- 1 Fig.
Appears in Collections:(EEAD) Artículos
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