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dc.contributor.authorBecana Ausejo, Manuel-
dc.contributor.authorSalin, Marvin L.-
dc.contributor.authorJi, Lin-
dc.contributor.authorKlucas, R. V.-
dc.date.accessioned2015-01-07T11:33:53Z-
dc.date.available2015-01-07T11:33:53Z-
dc.date.issued1991-03-
dc.identifier.citationPlanta 183 (4): 575-583 (1991)es_ES
dc.identifier.issn0032-0935-
dc.identifier.urihttp://hdl.handle.net/10261/109134-
dc.description22 Pags. The definitive version, with Figs., is available at: http://link.springer.com/journal/425es_ES
dc.description.abstractThe reduction of ferric leghemoglobin (Lb3+) from soybean (Glycine max (L.) Merr.) nodules by riboflavin, FMN and FAD in the presence of NAD(P)H was studied in vitro. The system NAD(P)H + flavin reduced Lb3+ to oxyferrous (Lb2+ · O2) or deoxyferrous (Lb2+) leghemoglobin in aerobic or anaerobic conditions, respectively. In the absence of O2 the reaction was faster and more effective (i.e. less NAD(P)H oxidized per mole Lb3+ reduced) than in the presence of O2; this phenomenon was probably because O2 competes with Lb3+ for reductant, thus generating activated O2 species. The flavin-mediated reduction of Lb3+ did not entail production of superoxide or peroxide, indicating that NAD(P)H-reduced flavins were able to reduce Lb3+ directly. The NAD(P)H + flavin system also reduced the complexes Lb3+ · nicotinate and Lb3+ · acetate to Lb2+ · O2, Lb2+ or Lb2+ · nicotinate, depending on the concentrations of ligands and of O2. In the presence of 200 μM nitrite most Lb remained as Lb3+ in aerobic conditions but the nitrosyl complex (Lb2+ · NO) was generated in anaerobic conditions. The above-mentioned characteristics of the NAD(P)H + flavin system, coupled with its effectiveness in reducing Lb3+ at physiological levels of NAD(P)H and flavins in soybean nodules, indicate that this mechanism may be especially important for reducing Lb3+ in vivo.es_ES
dc.description.sponsorshipM. Becana thanks the Spanish Ministry of Education and Science/Fulbright Commission for financial support.es_ES
dc.language.isoenges_ES
dc.publisherSpringeres_ES
dc.relation.isversionofPostprintes_ES
dc.rightsopenAccesses_ES
dc.subjectFlavines_ES
dc.subjectGlycine (nitrogen fixation)es_ES
dc.subjectLeghemoglobines_ES
dc.subjectNitrogen fixationes_ES
dc.subjectOxygen (activated)es_ES
dc.subjectPyridine nucleotideses_ES
dc.subjectSymbiosis (legume-Rhizobium)es_ES
dc.titleFlavin-mediated reduction of ferric leghemoglobin from soybean noduleses_ES
dc.typeartículoes_ES
dc.identifier.doi10.1007/BF00194279-
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionhttp://dx.doi.org/10.1007/BF00194279es_ES
dc.identifier.e-issn1432-2048-
dc.relation.csices_ES
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