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dc.contributor.authorManzano Román, Raúl-
dc.contributor.authorEncinas Grandes, Antonio-
dc.contributor.authorPérez Sánchez, Ricardo-
dc.identifier.citationVeterinary Parasitology 135(1): 65-79 (2006)en_US
dc.description15 pages, 5 figures, 5 tables.-- PMID: 16169153 [PubMed].-- Online version published on Oct 5, 2005.en_US
dc.description.abstractOrnithodoros erraticus is an argasid tick that can transmit severe diseases such as human relapsing fever and African swine fever. In southern Europe O. erraticus lives in close association with swine on free-range pig farms. Application of acaricides for the eradication of O. erraticus from pig farms is inefficient. This is the reason why we tried to develop an anti-O. erraticus vaccine as alternative method of control. Accordingly, we were prompted to investigate the protective possibilities of a midgut membrane extract from the parasite (GME) that has not been studied hitherto. Administration of the GME with Freund's adjuvants (FAs) to pigs and mice induced a protective response able to kill 80% of the immature forms of the parasite in the first 72 h post-feeding and to reduce the fecundity of females by more than 50%. The action of the vaccine is the result of damage to the midgut wall of the argasid, and, in mice, it has been shown that this damage is mediated by activation of the complement system. In pigs, the administration of GME with alum, instead of with FAs, reduced the degree of protection. The protective antigens of the GME were expressed by all the developmental stages examined and are probably proteins from the luminal membrane of midgut epithelial cells. These antigens were seen to be more abundant in recently fed parasites than in fasting specimens, suggesting that their expression is induced after blood ingestion.en_US
dc.description.sponsorshipThis work was supported by grant No. AGF99-0798 from the Spanish government and by grant No. QRLT-2000-02216 from the European Commission. We wish to express our acknowledgement to Dr. Ana Oleaga for her critical reading of the manuscript and helpful discussions.en_US
dc.format.extent22195 bytes-
dc.subjectOrnithodoros erraticusen_US
dc.subjectMidgut antigensen_US
dc.titleAntigens from the midgut membranes of Ornithodoros erraticus induce lethal anti-tick immune responses in pigs and miceen_US
dc.description.peerreviewedPeer revieweden_US
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