English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/10897
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

The unique proline of the Prochlorothrix hollandica plastocyanin hydrophobic patch impairs electron transfer to photosystem I

AuthorsNavarro, José A. ; Myshkin, Eugene; Rosa, Miguel A. de la ; Bullerjahn, George S.; Hervás, Manuel
Issue Date16-Jul-2001
PublisherAmerican Society for Biochemistry and Molecular Biology
CitationJournal of Biological Chemistry 276(40): 37501-37505 (2001)
AbstractA number of surface residues of plastocyanin from Prochlorothrix hollandica have been modified by site-directed mutagenesis. Changes have been made in amino acids located in the amino-terminal hydrophobic patch of the copper protein, which presents a variant structure as compared with other plastocyanins. The single mutants Y12G, Y12F, Y12W, P14L, and double mutant Y12G/P14L have been produced. Their reactivity toward photosystem I has been analyzed by laser flash absorption spectroscopy. Plots of the observed rate constant with all mutants versus plastocyanin concentration show a saturation profile similar to that with wild-type plastocyanin, thus suggesting the formation of a plastocyanin-photosystem I transient complex. The mutations do not induce relevant changes in the equilibrium constant for complex formation but induce significant variations in the electron transfer rate constant, mainly with the two mutants at proline 14. Additionally, molecular dynamics calculations indicate that mutations at position 14 yield small changes in the geometry of the copper center. The comparative kinetic analysis of the reactivity of plastocyanin mutants toward photosystem I from different organisms (plants and cyanobacteria) reveals that reversion of the unique proline of Prochlorothrix plastocyanin to the conserved leucine of all other plastocyanins at this position enhances the reactivity of the Prochlorothrix protein.
Description5 pages, 5 figures, 3 tables.-- PMID: 11457853 [PubMed].-- Printed version published on Oct 5, 2001.
Publisher version (URL)http://dx.doi.org/10.1074/jbc.M105367200
URIhttp://hdl.handle.net/10261/10897
DOI10.1074/jbc.M105367200
ISSN0021-9258
Appears in Collections:(IBVF) Artículos
Files in This Item:
There are no files associated with this item.
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.