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Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/108821
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dc.contributor.authorMajer, Eszter-
dc.contributor.authorSalvador, Zaira-
dc.contributor.authorZwart, Mark P.-
dc.contributor.authorWillemsen, Anouk-
dc.contributor.authorElena, Santiago F.-
dc.contributor.authorDaròs Arnau, José Antonio-
dc.date.accessioned2014-12-17T09:39:09Z-
dc.date.available2014-12-17T09:39:09Z-
dc.date.issued2014-04-01-
dc.identifier.citationJournal of Virology (88): 4586-4590 (2014)es_ES
dc.identifier.issn0022-538X-
dc.identifier.urihttp://hdl.handle.net/10261/108821-
dc.description4 Figurases_ES
dc.description.abstractPotyviruses express most of their proteins from a long open reading frame that is translated into a large polyprotein processed by three viral proteases. To understand the constraints on potyvirus genome organization, we relocated the viral RNA-dependent RNA polymerase (NIb) cistron to all possible intercistronic positions of the Tobacco etch virus (TEV) polyprotein. Only viruses with NIb at the amino terminus of the polyprotein or in between P1 and HC-Pro were viable in tobacco plants.es_ES
dc.description.sponsorshipWe thank Verónica Aragonés for excellent technical assistance. This research was supported by grant BIO2011-26741 from the Spanish Ministerio de Economía y Competitividad (MINECO) to J.-A.D., grant PROMETEO/2010/019 from Generalitat Valenciana to S.F.E. and J.-A.D., and grants BFU2012-30805 and 22371 from MINECO and the John Templeton Foundation, respectively, to S.F.E. E.M. was supported by a predoctoral fellowship (AP2012-3751) from the Spanish Ministerio de Educación, Cultura y Deporte. M.P.Z. was supported by a Juan de la Cierva postdoctoral contract (JCI-2011-10379) from MINECO and a Rubicon grant from the Netherlands Organization for Scientific Research (www.nwo.nl). The opinions expressed in this publication are those of the authors and do not necessarily reflect the views of the John Templeton Foundation.es_ES
dc.language.isoenges_ES
dc.publisherAmerican Society for Microbiologyes_ES
dc.relation.isversionofPreprintes_ES
dc.rightsopenAccesses_ES
dc.subjectVPG-PROTEINASE NIAes_ES
dc.subjectHETEROLOGOUS PROTEINSes_ES
dc.subjectVIRUS GENOMEes_ES
dc.subjectEXPRESSIONes_ES
dc.subjectSTABILITYes_ES
dc.subjectVECTORes_ES
dc.subjectPLANTSes_ES
dc.subjectINSERTIONes_ES
dc.subjectMUTANTSes_ES
dc.subjectPURIFICATIONes_ES
dc.titleRelocation of the NIb Gene in the Tobacco Etch Potyvirus Genomees_ES
dc.typeartículoes_ES
dc.identifier.doi10.1128/JVI.03336-13-
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionhttp://dx.doi.org/10.1128/JVI.03336-13es_ES
dc.identifier.e-issn1098-5514-
dc.relation.csicSíes_ES
dc.identifier.pmid24453370-
dc.type.coarhttp://purl.org/coar/resource_type/c_6501es_ES
item.openairetypeartículo-
item.grantfulltextopen-
item.cerifentitytypePublications-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextWith Fulltext-
item.languageiso639-1en-
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