English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/108788
Compartir / Impacto:
Estadísticas
Add this article to your Mendeley library MendeleyBASE
 |  Ver citas en Google académico
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar otros formatos: Exportar EndNote (RIS)Exportar EndNote (RIS)Exportar EndNote (RIS)
Título

Structural basis for DNA binding specificity by the auxin-dependent ARF transcription factors

Autor Boer, Roeland ; López-Vidriero, Irene; Franco-Zorrilla, José Manuel; Coll, Miquel
Palabras clave auxin response factor
homodimer
monomer
transcription factor
unclassified drug
adenosine diphosphate ribosylation factor 5
adenosine diphosphate ribosylation factor 1
auxin
ARF protein
oligonucleotide
Fecha de publicación 30-ene-2014
EditorCell Press
Citación Cell 156(3): 577-589 (2014)
ResumenAuxin regulates numerous plant developmental processes by controlling gene expression via a family of functionally distinct DNA-binding auxin response factors (ARFs), yet the mechanistic basis for generating specificity in auxin response is unknown. Here, we address this question by solving high-resolution crystal structures of the pivotal Arabidopsis developmental regulator ARF5/MONOPTEROS (MP), its divergent paralog ARF1, and a complex of ARF1 and a generic auxin response DNA element (AuxRE). We show that ARF DNA-binding domains also homodimerize to generate cooperative DNA binding, which is critical for in vivo ARF5/MP function. Strikingly, DNA-contacting residues are conserved between ARFs, and we discover that monomers have the same intrinsic specificity. ARF1 and ARF5 homodimers, however, differ in spacing tolerated between binding sites. Our data identify the DNA-binding domain as an ARF dimerization domain, suggest that ARF dimers bind complex sites as molecular calipers with ARF-specific spacing preference, and provide an atomic-scale mechanistic model for specificity in auxin response. © 2014 Elsevier Inc.
Descripción Boer, Roeland et al.
Versión del editorhttp://dx.doi.org/10.1016/j.cell.201312.027
URI http://hdl.handle.net/10261/108788
DOI10.1016/j.cell.201312.027
Identificadoresdoi: 10.1016/j.cell.2013.12.027
issn: 0092-8674
Aparece en las colecciones: (IBMB) Artículos
(CNB) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
accesoRestringido.pdf15,38 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.