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Título

Crystal structure of a DNA Holliday junction

Autor Ortiz-Lombardía, Miguel; González, Ana ; Eritja Casadellà, Ramón; Aymamí, Joan; Azorín, Ferran ; Coll, Miquel
Palabras clave priority journal
stereospecificity
molecular interaction
isomerism
hydrogen bond
base mispairing
conformational transition
crystal structure
DNA helix
DNA recombination
DNA structure
holliday junction
Fecha de publicación 1999
EditorNature Publishing Group
Citación Nature Structural Biology 6(10): 913-917 (1999)
ResumenDNA recombination is a universal biological event responsible both for the generation of genetic diversity and for the maintenance of genome integrity. A four-way DNA junction, also termed Holliday junction, is the key intermediate in nearly all recombination processes. This junction is the substrate of recombination enzymes that promote branch migration or catalyze its resolution. We have determined the crystal structure of a four-way DNA junction by multiwavelength anomalous diffraction, and refined it to 2.16 Å resolution. The structure has two-fold symmetry, with pairwise stacking of the double-helical arms, which form two continuous B-DNA helices that run antiparallel, cross in a right-handed way, and contain two G-A mismatches. The exchanging backbones form a compact structure with strong van der Waals contacts and hydrogen bonds, implying that a conformational change must occur for the junction to branch-migrate or isomerize. At the branch point, two phosphate groups from one helix occupy the major groove of the other one, establishing sequence-specific hydrogen bonds. These interactions, together with different stacking energies and steric hindrances, explain the preference for a particular junction stacked conformer.
Versión del editorhttp://dx.doi.org/10.1038/13277
URI http://hdl.handle.net/10261/108726
DOI10.1038/13277
Identificadoresissn: 1072-8368
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