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Título

Establishment and development of intestinal microbiota in preterm neonates. A posible target for the probiotic action

Autor Arboleya, Silvia ; Salazar, Nuria ; Fernández, Nuria; Solís, Gonzalo; Margolles Barros, Abelardo ; Hernández-Barranco, Ana María ; González de los Reyes-Gavilán, Clara ; Gueimonde Fernández, Miguel
Fecha de publicación may-2012
Citación 2nd Workshop of the European Network for Gastrointestinal Health Research (2012)
ResumenMicrobial colonization of the infant gut plays an essential role in the development of the intestine and the immune system of newborns. The intestinal microbiota of full-term breast-fed (FTBF) infants is currently considered as the health standard for newborns. In contrast, the immature intestine, the frequent use of antibiotics and formula milk and the long stay at hospitals jeopardize the proper microbiota development in premature infants. The establishment of the gut microbiota in preterm neonates during the %rst three months of life was assessed and compared with that of FTBF infants. Microbial composition was determined in faeces by qPCR, and metagenomic analyses; short chain fatty acids (SCFA) were quanti%ed by Gas-Chromatography-Flame Injection Detector/Mass Spectrometry (GC-FID/MS). All techniques allowed clearly di!erentiating preterm from FTBF infants. Premature infants showed higher levels of facultative anaerobes and lower levels of anaerobes such as Bi!dobacterium , Bacteroides and Atopobium as well as lower levels of SCFA during the %rst days of life. The deep alterations found in the process of microbiota establishment in preterm infants, indicated the need for intervention strategies to counteract them. Then, 16 Bi!dobacterium strains were tested in fecal batch slurry cultures from preterm babies for their ability to modulate in vitro the intestinal microbiota. Those bi!dobacteria that in fecal cultures counteracted better the aberrancies previously found in feces of preterm babies as compared with FTBF infants, were selected. Three Bi!dobacterium bi!dum strains from infant feces (IF23/, IF10/10, and IF10/20) as well as two Bi!dobacterium breve strains from breast milk (BM 13/14 and BM 23/20) promoted the most suitable shift in SCFA pro%les and in the population of facultative anaerobes and anaerobes, representing promising candidates for further in vitro and in vivo studies.
Descripción Trabajo presentado en la 2nd Workshop of the European Network for Gastrointestinal Health Research, ENGIHR (Diet and the gut microbiota: new directions), celebrada en Helsinki del 2 al 4 de mayo de 2012.
URI http://hdl.handle.net/10261/108500
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