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Crystallization and preliminary X-ray analysis of the DNA decamers d(CCGGATCCGG) and d(CCGGCGCCGG)

AuthorsAymamí, Joan; Pous, Joan ; Coll, Miquel ; Lisgarten, John N.
KeywordsDNA structure
Issue DateFeb-2002
PublisherBlackwell Publishing
CitationActa Crystallographica. Section D: Biological Crystallography 58(2): 310-311 (2002)
AbstractThe DNA decamers d(CCGGATCCGG) and d(CCGGCGCCGG) have been crystallized for X-ray analysis in order to investigate the effects of changing the two central base pairs of the DNA fragment d(CCGGGACCGG). Previous studies have already demonstrated that the structure of the former DNA fragment contains a DNA Holliday junction. Crystals were obtained at 293 K by the hanging-drop vapour-diffusion technique using the Nucleic Acid Mini Screen. Over a period of two weeks, hexagonal plates appeared. For the DNA fragment d(CCGGATCCGG), the crystals belong to space group P31, with unit-cell parameters a = b = 33.54, c = 46.39 Å, α = β = 90, γ = 120°, and diffract to 2.2 Å. In the case of the DNA fragment d(CCGGCGCCGG) the crystals belong to the space group C2, with unit-cell parameters a = 65.35, b = 24.07, c = 37.34 Å, β = 109.97°, and diffract to 2.0 Å
Publisher version (URL)http://dx.doi.org/10.1107/S0907444901018959
Identifiersdoi: 10.1107/S0907444901018959
issn: 0907-4449
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