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Título

Crystallization and preliminary X-ray analysis of the DNA decamers d(CCGGATCCGG) and d(CCGGCGCCGG)

Autor Aymamí, Joan; Pous, Joan; Coll, Miquel ; Lisgarten, John N.
Palabras clave DNA structure
Fecha de publicación feb-2002
EditorBlackwell Publishing
Citación Acta Crystallographica. Section D: Biological Crystallography 58(2): 310-311 (2002)
ResumenThe DNA decamers d(CCGGATCCGG) and d(CCGGCGCCGG) have been crystallized for X-ray analysis in order to investigate the effects of changing the two central base pairs of the DNA fragment d(CCGGGACCGG). Previous studies have already demonstrated that the structure of the former DNA fragment contains a DNA Holliday junction. Crystals were obtained at 293 K by the hanging-drop vapour-diffusion technique using the Nucleic Acid Mini Screen. Over a period of two weeks, hexagonal plates appeared. For the DNA fragment d(CCGGATCCGG), the crystals belong to space group P31, with unit-cell parameters a = b = 33.54, c = 46.39 Å, α = β = 90, γ = 120°, and diffract to 2.2 Å. In the case of the DNA fragment d(CCGGCGCCGG) the crystals belong to the space group C2, with unit-cell parameters a = 65.35, b = 24.07, c = 37.34 Å, β = 109.97°, and diffract to 2.0 Å
Versión del editorhttp://dx.doi.org/10.1107/S0907444901018959
URI http://hdl.handle.net/10261/108280
DOI10.1107/S0907444901018959
Identificadoresdoi: 10.1107/S0907444901018959
issn: 0907-4449
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