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Título

Giant vesicles: A powerful tool to reconstruct bacterial division assemblies in cell-like compartments

Autor Jiménez, Mercedes ; Martos, Ariadna ; Raso, A; Rivas, Germán
Palabras clave Bacteria
Bacterial Proteins
Lipids
Unilamellar Liposomes
Cell Division
Fecha de publicación 2013
EditorBlackwell Publishing
Citación Environmental Microbiology 15: 3158- 3168 (2013)
ResumenThe use of artificial lipid membranes, structured as giant unilamellar vesicles (GUVs), provides the opportunity to investigate membrane-associated biological processes under defined experimental conditions. Due to their large size, they are uniquely adapted to investigate the properties and organization (in time and space) of macromolecular complexes incorporated in the vesicle interior by imaging and micro-spectroscopic techniques. Experimental methods to produce giant vesicles and to encapsulate proteins inside them are here reviewed. Previous experimental work to reconstitute elements of the bacterial division machinery in these membrane-like systems is summarized. Future challenges towards reconstructing minimal divisome assemblies in giant vesicles as cytomimetic containers are discussed. © 2013 Society for Applied Microbiology and John Wiley & Sons Ltd.
URI http://hdl.handle.net/10261/108277
DOIhttp://dx.doi.org/10.1111/1462-2920.12214
ISSN1462-2912
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