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Title

Application of the use of high-throughput technologies to the determination of protein structures of bacterial and viral pathogens

AuthorsFogg, Mark J.; Coll, Miquel
KeywordsAutomation
Bacteria
Viruses
Expression
Miniaturization
Cloning
Issue Date2006
PublisherBlackwell Publishing
CitationActa Crystallographica Section D: Biological Crystallography 62(10): 1196-1207 (2006)
AbstractThe Structural Proteomics In Europe (SPINE) programme is aimed at the development and implementation of high-throughput technologies for the efficient structure determination of proteins of biomedical importance, such as those of bacterial and viral pathogens linked to human health. Despite the challenging nature of some of these targets, 175 novel pathogen protein structures (∼220 including complexes) have been determined to date. Here the impact of several technologies on the structural determination of proteins from human pathogens is illustrated with selected examples, including the parallel expression of multiple constructs, the use of standardized refolding protocols and optimized crystallization screens. © International Union of Crystallography, 2006.
DescriptionFogg, Mark J. et al.
Publisher version (URL)http://dx.doi.org/10.1107/S0907444906030915
URIhttp://hdl.handle.net/10261/108270
DOIhttp://dx.doi.org/10.1107/S0907444906030915
Identifiersdoi: 10.1107/S0907444906030915
issn: 0907-4449
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