English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/108155
COMPARTIR / IMPACTO:
Estadísticas
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Título

Crystallization and preliminary X-ray diffraction studies of the transcriptional repressor PaaX, the main regulator of the phenylacetic acid degradation pathway in Escherichia coli W

AutorRojas-Altuve, A.; Carrasco-López, César ; Hernández-Rocamora, V. M. ; Sanz, Jesús M. ; Hermoso, Juan A.
Fecha de publicación1-oct-2011
EditorInternational Union of Crystallography
CitaciónActa Cryst. (2011). F67, 1278–1280
ResumenPaaX is the main regulator of the phenylacetic acid aerobic degradation pathway in bacteria and acts as a transcriptional repressor in the absence of its inducer phenylacetyl-coenzyme A. The natural presence and the recent accumulation of a variety of highly toxic aromatic compounds owing to human pollution has created considerable interest in the study of degradation pathways in bacteria, the most important microorganisms capable of recycling these compounds, in order to design and apply novel bioremediation strategies. PaaX from Escherichia coliWwas cloned, overexpressed, purified and crystallized using the sitting-drop vapour-diffusion method at 291 K. Crystals grew from a mixture of 0.9 MLi2SO4 and 0.5 Msodium citrate pH 5.8. These crystals, which belonged to the monoclinic space group C2 with unit-cell parameters a = 167.88, b = 106.23, c = 85.87 A ° , = 108.33 , allowed the collection of an X-ray data set to 2.3 A ° resolution.
Descripción3 p.-2 fig.-1 tab.
Versión del editorhttp://dx.doi.org/10.1107/S1744309111029873
URIhttp://hdl.handle.net/10261/108155
DOI10.1107/S1744309111029873
ISSN1744-3091
Aparece en las colecciones: (CIB) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
Acta Cristalographica F. 2011, 67 (10) 278-.pdf272,32 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.