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UV laser desorption/ionization of polymers and peptides assisted by platinum nanoparticles synthesized by laser ablation in water

AutorCueto, Maite ; Gámez, F.; Hortal, A. R.; Hurtado, P.; Martínez-Haya, Bruno; Sanz, M. ; Oujja, M.; Castillejo, Marta
Fecha de publicaciónfeb-2013
EditorUniversidad Complutense de Madrid
CitaciónV Jornadas de Jóvenes Investigadores en Fí sica At ómica y Molecular (J2IFAM 2013), February, 13rd-15th 2013, Madrid
ResumenWe have investigated benchmark laser desorption/ionization mass spectroscopy processes assisted by platinum nanoparticles (NALDI-MS). The technique has been applied to the detection of a synthetic polymer (polyethylenglycol PEG600) and a peptide (Angiotensin I). Platinum stands out among the noble metals as a NALDI active substrate because of its low heat conductivity and high melting temperature [1]. It has been shown that stable platinum nanoparticles suitable for NALDI can be prepared by laser ablation in water solutions [2,3]. In this work, we use aqueous suspensions of platinum colloidal nanoparticles of varying size, shape, cristallinity and polydispersity produced in solution by ablation with a Q-switched Nd:YAG laser at several laser wavelengths (1064, 532 and 266 nm) and stabilizing agents, such as PEG, PVA, PVP and citrate. NALDI-MS measurements in positive-ion mode were performed in a commercial time-of-flight mass spectrometer (UltraeXtreme, Bruker), employing 355 nm nanosecond laser pulses from a Nd:YAG laser at a 1 kHz repetition rate. Good sensitivity is obtained for the detection of both PEG600 and Angiotensin I. For PEG600 the LDI spectra display adducts associated with the cationization by Na+ or K+, whereas for the Angiotensin the protonated peptide is detected along with the cationized adducts, with relative intensities depending on the surfactant employed. The best overall NALDI-MS performance was found for nanoparticles of average size 4 nm, produced with 532 nm laser pulses in the presence of sodium citrate as surfactant.
DescripciónFEDER-Andalucia2007-2013 through projects P07-FQM-02600 and P09-FQM-4938 . Funding from MICINN under Projects CTQ2010-15680 and CONSOLIDER CSD2007-00058, and Programa Geomateriales (CAM, S2009/Mat-1629).
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