English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/108037
Compartir / Impacto:
Estadísticas
Add this article to your Mendeley library MendeleyBASE
Ver citas en Google académico
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar otros formatos: Exportar EndNote (RIS)Exportar EndNote (RIS)Exportar EndNote (RIS)
Título

UV laser desorption/ionization of polymers and peptides assisted by platinum nanoparticles synthesized by laser ablation in water

Autor Cueto, Maite ; Gámez, F.; Hortal, A.R.; Hurtado, P.; Martínez-Haya, B.; Sanz, M. ; Oujja, M.; Castillejo, Marta
Fecha de publicación feb-2013
EditorUniversidad Complutense de Madrid
Citación V Jornadas de Jóvenes Investigadores en Fí sica At ómica y Molecular (J2IFAM 2013), February, 13rd-15th 2013, Madrid
ResumenWe have investigated benchmark laser desorption/ionization mass spectroscopy processes assisted by platinum nanoparticles (NALDI-MS). The technique has been applied to the detection of a synthetic polymer (polyethylenglycol PEG600) and a peptide (Angiotensin I). Platinum stands out among the noble metals as a NALDI active substrate because of its low heat conductivity and high melting temperature [1]. It has been shown that stable platinum nanoparticles suitable for NALDI can be prepared by laser ablation in water solutions [2,3]. In this work, we use aqueous suspensions of platinum colloidal nanoparticles of varying size, shape, cristallinity and polydispersity produced in solution by ablation with a Q-switched Nd:YAG laser at several laser wavelengths (1064, 532 and 266 nm) and stabilizing agents, such as PEG, PVA, PVP and citrate. NALDI-MS measurements in positive-ion mode were performed in a commercial time-of-flight mass spectrometer (UltraeXtreme, Bruker), employing 355 nm nanosecond laser pulses from a Nd:YAG laser at a 1 kHz repetition rate. Good sensitivity is obtained for the detection of both PEG600 and Angiotensin I. For PEG600 the LDI spectra display adducts associated with the cationization by Na+ or K+, whereas for the Angiotensin the protonated peptide is detected along with the cationized adducts, with relative intensities depending on the surfactant employed. The best overall NALDI-MS performance was found for nanoparticles of average size 4 nm, produced with 532 nm laser pulses in the presence of sodium citrate as surfactant.
Descripción FEDER-Andalucia2007-2013 through projects P07-FQM-02600 and P09-FQM-4938 . Funding from MICINN under Projects CTQ2010-15680 and CONSOLIDER CSD2007-00058, and Programa Geomateriales (CAM, S2009/Mat-1629).
URI http://hdl.handle.net/10261/108037
Aparece en las colecciones: (IQFR) Comunicaciones congresos
(CFMAC-IEM) Comunicaciones congresos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
Poster_definitivo_VJornadasJovenesInvestigadores.pdf2,62 MBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.