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A comparative study of the cytometric characteristics of High and Low nucleic-acid bacterioplankton cells from different aquatic ecosystems

AutorBouvier, Thierry; Del Giorgio, Paul A.; Gasol, Josep M.
Fecha de publicaciónago-2007
EditorSociety for Applied Microbiology
CitaciónEnvironmental Microbiology 9(8): 2050-2066 (2007)
ResumenFlow cytometry has revealed the existence of two distinct fractions of bacterioplankton cells, characterized by high and low nucleic acid contents (HNA and LNA cells). Although these fractions seem ubiquitous in aquatic systems, little is known concerning the variation in the cytometric parameters used to characterize them. We have performed cytometric analyses of samples from a wide range of aquatic systems to determine the magnitude and variability in the cytometric characteristics of HNA/LNA. We show that neither group is associated to a fixed level of fluorescence and of light scatter. Rather, the relative position of HNA and LNA in the fluorescence versus side scatter cytograms varies greatly, both within and among ecosystems. Although the cytometric parameters of both groups tend to covary, there is often uncoupling between the two, particularly in light scatter. Our results show that, although the basic HNA/LNA configuration is present in most samples, its cytometric expression changes greatly in different ecosystems and along productivity gradients. The patterns in cytometric parameters do not support the simple, dichotomous view of HNA and LNA as active and inactive cells, or the notion of two distinct and independent communities, but rather suggest that there may be cells that are intrinsic to each fraction, as well as others that may exchange between fractions. © 2007 The Authors
Descripción17 pages, 8 figures, 5 tables
Versión del editorhttp://dx.doi.org/10.1111/j.1462-2920.2007.01321.x
Identificadoresdoi: 10.1111/j.1462-2920.2007.01321.x
issn: 1462-2912
e-issn: 1462-2920
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