English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/10762
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Exportar a otros formatos:


Spectroscopic study of the interaction of actinomycin D with oligonucleotides carrying the central base sequences -XGCY- and -XGGCCY- using multivariate methods

AuthorsVives Blázquez, Montse; Eritja Casadellà, Ramón ; Tauler, Romà
KeywordsActinomycin D
Multivariate analysis
Multivariate curve resolution
Issue DateJan-2007
CitationAnalytical and Bioanalytical Chemistry 387(1): 311-320 (2007)
AbstractThe interactions of actinomycin D (ACTD) with the oligonucleotides 5′-CAAAGCTTTG-3′, 5′-CATGGC CATG-3′ and 5′-TATGGCCATA-3′ were investigated by means of acid–base titrations and mole-ratio and melting experiments monitored by molecular absorption and circular dichroism (CD) spectroscopies. For each experiment, CD and molecular absorption spectra were recorded at each point in the experiment, and later analyzed via appropriate multivariate data analysis methods. The study of the interactions between these oligonucleotides and ACTD at 25°C showed the formation of an interaction complex with a stoichiometry of 1:1 (ACTD:duplex) and values for the log(formation constant) of 5.1 ± 0.3, 6.4 ± 0.2, and 5.6 ± 0.2, respectively. An additional interaction complex at higher temperatures was also detected, which might be related to the single-stranded forms of the oligonucleotides.
Description10 pages, 5 figures, 1 table.-- PMID: 17123067 [PubMed].-- Published online Nov 23, 2006.
Supporting information (4 pages, 3 suppl. figures) available at: http://www.springerlink.com/content/870650451902431q/MediaObjects/216_2006_946_MOESM1_ESM.doc
Publisher version (URL)http://dx.doi.org/10.1007/s00216-006-0946-8
Appears in Collections:(IDAEA) Artículos
(IBMB) Artículos
Files in This Item:
File Description SizeFormat 
spectroscopic_study_Vives.pdf372,47 kBAdobe PDFThumbnail
Show full item record
Review this work

Related articles:

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.