English
español
Please use this identifier to cite or link to this item:
http://hdl.handle.net/10261/10762
Share/Impact:
Statistics |
![]() ![]() ![]() |
|
|
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |||
|
Title: | Spectroscopic study of the interaction of actinomycin D with oligonucleotides carrying the central base sequences -XGCY- and -XGGCCY- using multivariate methods |
Authors: | Vives Blázquez, Montse; Eritja Casadellà, Ramón ![]() ![]() |
Keywords: | Actinomycin D Multivariate analysis Oligonucleotides Spectroscopy Multivariate curve resolution |
Issue Date: | Jan-2007 |
Publisher: | Springer |
Citation: | Analytical and Bioanalytical Chemistry 387(1): 311-320 (2007) |
Abstract: | The interactions of actinomycin D (ACTD) with the oligonucleotides 5′-CAAAGCTTTG-3′, 5′-CATGGC CATG-3′ and 5′-TATGGCCATA-3′ were investigated by means of acid–base titrations and mole-ratio and melting experiments monitored by molecular absorption and circular dichroism (CD) spectroscopies. For each experiment, CD and molecular absorption spectra were recorded at each point in the experiment, and later analyzed via appropriate multivariate data analysis methods. The study of the interactions between these oligonucleotides and ACTD at 25°C showed the formation of an interaction complex with a stoichiometry of 1:1 (ACTD:duplex) and values for the log(formation constant) of 5.1 ± 0.3, 6.4 ± 0.2, and 5.6 ± 0.2, respectively. An additional interaction complex at higher temperatures was also detected, which might be related to the single-stranded forms of the oligonucleotides. |
Description: | 10 pages, 5 figures, 1 table.-- PMID: 17123067 [PubMed].-- Published online Nov 23, 2006. Supporting information (4 pages, 3 suppl. figures) available at: http://www.springerlink.com/content/870650451902431q/MediaObjects/216_2006_946_MOESM1_ESM.doc |
Publisher version (URL): | http://dx.doi.org/10.1007/s00216-006-0946-8 |
URI: | http://hdl.handle.net/10261/10762 |
DOI: | 10.1007/s00216-006-0946-8 |
ISSN: | 1618-2642 |
E-ISSN: | 1618-2650 |
Appears in Collections: | (IDAEA) Artículos (IBMB) Artículos |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
spectroscopic_study_Vives.pdf | 372,47 kB | Adobe PDF | ![]() View/Open |
Show full item record
Review this work
Review this work
Related articles:
WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.