Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/10762
Share/Export:
logo share SHARE logo core CORE BASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Title

Spectroscopic study of the interaction of actinomycin D with oligonucleotides carrying the central base sequences -XGCY- and -XGGCCY- using multivariate methods

AuthorsVives Blázquez, Montse; Eritja Casadellà, Ramón CSIC ORCID ; Tauler, Romà
KeywordsActinomycin D
Multivariate analysis
Oligonucleotides
Spectroscopy
Multivariate curve resolution
Issue DateJan-2007
PublisherSpringer
CitationAnalytical and Bioanalytical Chemistry 387(1): 311-320 (2007)
AbstractThe interactions of actinomycin D (ACTD) with the oligonucleotides 5′-CAAAGCTTTG-3′, 5′-CATGGC CATG-3′ and 5′-TATGGCCATA-3′ were investigated by means of acid–base titrations and mole-ratio and melting experiments monitored by molecular absorption and circular dichroism (CD) spectroscopies. For each experiment, CD and molecular absorption spectra were recorded at each point in the experiment, and later analyzed via appropriate multivariate data analysis methods. The study of the interactions between these oligonucleotides and ACTD at 25°C showed the formation of an interaction complex with a stoichiometry of 1:1 (ACTD:duplex) and values for the log(formation constant) of 5.1 ± 0.3, 6.4 ± 0.2, and 5.6 ± 0.2, respectively. An additional interaction complex at higher temperatures was also detected, which might be related to the single-stranded forms of the oligonucleotides.
Description10 pages, 5 figures, 1 table.-- PMID: 17123067 [PubMed].-- Published online Nov 23, 2006.
Supporting information (4 pages, 3 suppl. figures) available at: http://www.springerlink.com/content/870650451902431q/MediaObjects/216_2006_946_MOESM1_ESM.doc
Publisher version (URL)http://dx.doi.org/10.1007/s00216-006-0946-8
URIhttp://hdl.handle.net/10261/10762
DOI10.1007/s00216-006-0946-8
ISSN1618-2642
E-ISSN1618-2650
Appears in Collections:(IDAEA) Artículos
(IBMB) Artículos

Files in This Item:
File Description SizeFormat
spectroscopic_study_Vives.pdf372,47 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work

SCOPUSTM   
Citations

13
checked on May 10, 2022

WEB OF SCIENCETM
Citations

12
checked on May 15, 2022

Page view(s)

454
checked on May 16, 2022

Download(s)

255
checked on May 16, 2022

Google ScholarTM

Check

Altmetric

Dimensions


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.