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Título

Spectroscopic study of the interaction of actinomycin D with oligonucleotides carrying the central base sequences -XGCY- and -XGGCCY- using multivariate methods

AutorVives Blázquez, Montse; Eritja Casadellà, Ramón; Tauler, Romà
Palabras claveActinomycin D
Multivariate analysis
Oligonucleotides
Spectroscopy
Multivariate curve resolution
Fecha de publicaciónene-2007
EditorSpringer
CitaciónAnalytical and Bioanalytical Chemistry 387(1): 311-320 (2007)
ResumenThe interactions of actinomycin D (ACTD) with the oligonucleotides 5′-CAAAGCTTTG-3′, 5′-CATGGC CATG-3′ and 5′-TATGGCCATA-3′ were investigated by means of acid–base titrations and mole-ratio and melting experiments monitored by molecular absorption and circular dichroism (CD) spectroscopies. For each experiment, CD and molecular absorption spectra were recorded at each point in the experiment, and later analyzed via appropriate multivariate data analysis methods. The study of the interactions between these oligonucleotides and ACTD at 25°C showed the formation of an interaction complex with a stoichiometry of 1:1 (ACTD:duplex) and values for the log(formation constant) of 5.1 ± 0.3, 6.4 ± 0.2, and 5.6 ± 0.2, respectively. An additional interaction complex at higher temperatures was also detected, which might be related to the single-stranded forms of the oligonucleotides.
Descripción10 pages, 5 figures, 1 table.-- PMID: 17123067 [PubMed].-- Published online Nov 23, 2006.
Supporting information (4 pages, 3 suppl. figures) available at: http://www.springerlink.com/content/870650451902431q/MediaObjects/216_2006_946_MOESM1_ESM.doc
Versión del editorhttp://dx.doi.org/10.1007/s00216-006-0946-8
URIhttp://hdl.handle.net/10261/10762
DOI10.1007/s00216-006-0946-8
ISSN1618-2642
E-ISSN1618-2650
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