English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/107510
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:


ExpR Is Not Required for Swarming but Promotes Sliding in Sinorhizobium meliloti

AuthorsNogales, Joaquina; Bernabéu-Roda, Lydia; Cuellar, Virginia; Soto, María José
Issue DateApr-2012
PublisherAmerican Society for Microbiology
CitationJournal of Bacteriology 194(8): 2027-2035 (2012)
AbstractSwarming is a mode of translocation dependent on flagellar activity that allows bacteria to move rapidly across surfaces. In several bacteria, swarming is a phenotype regulated by quorum sensing. It has been reported that the swarming ability of the soil bacterium Sinorhizobium meliloti Rm2011 requires a functional ExpR/Sin quorum-sensing system. However, our previous published results demonstrate that strains Rm1021 and Rm2011, both known to have a disrupted copy of expR, are able to swarm on semisolid minimal medium. In order to clarify these contradictory results, the role played by the LuxR-type regulator ExpR has been reexamined. Results obtained in this work revealed that S. meliloti can move over semisolid surfaces using at least two different types of motility. One type is flagellum-independent surface spreading or sliding, which is positively influenced by a functional expR gene mainly through the production of exopolysaccharide II (EPS II). To a lesser extent, EPS II-deficient strains can also slide on surfaces by a mechanism that is at least dependent on the siderophore rhizobactin 1021. The second type of surface translocation shown by S. meliloti is swarming, which is greatly dependent on flagella and rhizobactin 1021 but does not require ExpR. We have extended our study to demonstrate that the production of normal amounts of succinoglycan (EPS I) does not play a relevant role in surface translocation but that its overproduction facilitates both swarming and sliding motilities.
Publisher version (URL)http://dx.doi.org/10.1128/JB.06524-11
Identifiersissn: 0021-9193
Appears in Collections:(EEZ) Artículos
Files in This Item:
File Description SizeFormat 
ExpR_swarming_Nogales.pdf8,95 MBAdobe PDFThumbnail
Show full item record
Review this work

Related articles:

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.