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Título: | Enzymatic synthesis of disaccharides by β-galactosidase-catalyzed glycosylation of a glycocluster |
Autor: | Santiago, Reyes de CSIC; Fernández-Mayoralas, Alfonso CSIC ORCID ; García-Junceda, Eduardo CSIC ORCID | Palabras clave: | Enzymatic synthesis Liquid-phase synthesis Glycocluster β-galactosidase Glycosidase catalyzed transglycosylation |
Fecha de publicación: | 16-oct-2000 | Editor: | Elsevier | Citación: | Journal of Molecular Catalysis B: Enzymatic 11(2-3): 71-79 (2000) | Resumen: | The synthesis of disaccharides by galactosylation of a glucose-containing cluster has been studied using β-galactosidases from different sources. Galactosylation of the cluster could be detected by ESI-MS. After size-exclusion chromatography of the reaction mixtures and subsequent cleavage of the linker, galactopyranosyl-glucose disaccharides were obtained with yield and regioselectivity dependent on the origin of the enzyme. The possibility of using this method for the synthesis of multivalent glycoconjugates is discussed. | Descripción: | 9 pages, 2 figures, 3 schemes.-- Printed version published Dec 12, 2000. | Versión del editor: | http://dx.doi.org/10.1016/S1381-1177(00)00194-6 | URI: | http://hdl.handle.net/10261/10671 | DOI: | 10.1016/S1381-1177(00)00194-6 | ISSN: | 1381-1177 |
Aparece en las colecciones: | (IQOG) Artículos |
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Fichero | Descripción | Tamaño | Formato | |
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Glycosylation of a glycocluster.pdf | 227,15 kB | Adobe PDF | Visualizar/Abrir |
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