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Título

Enzymatic synthesis of disaccharides by β-galactosidase-catalyzed glycosylation of a glycocluster

AutorSantiago, Reyes de; Fernández-Mayoralas, Alfonso ; García-Junceda, Eduardo
Palabras claveEnzymatic synthesis
Liquid-phase synthesis
Glycocluster
β-galactosidase
Glycosidase catalyzed transglycosylation
Fecha de publicación16-oct-2000
EditorElsevier
CitaciónJournal of Molecular Catalysis B: Enzymatic 11(2-3): 71-79 (2000)
ResumenThe synthesis of disaccharides by galactosylation of a glucose-containing cluster has been studied using β-galactosidases from different sources. Galactosylation of the cluster could be detected by ESI-MS. After size-exclusion chromatography of the reaction mixtures and subsequent cleavage of the linker, galactopyranosyl-glucose disaccharides were obtained with yield and regioselectivity dependent on the origin of the enzyme. The possibility of using this method for the synthesis of multivalent glycoconjugates is discussed.
Descripción9 pages, 2 figures, 3 schemes.-- Printed version published Dec 12, 2000.
Versión del editorhttp://dx.doi.org/10.1016/S1381-1177(00)00194-6
URIhttp://hdl.handle.net/10261/10671
DOI10.1016/S1381-1177(00)00194-6
ISSN1381-1177
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