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Title

Enzymatic synthesis of disaccharides by β-galactosidase-catalyzed glycosylation of a glycocluster

AuthorsSantiago, Reyes de; Fernández-Mayoralas, Alfonso CSIC ORCID ; García-Junceda, Eduardo CSIC ORCID
KeywordsEnzymatic synthesis
Liquid-phase synthesis
Glycocluster
β-galactosidase
Glycosidase catalyzed transglycosylation
Issue Date16-Oct-2000
PublisherElsevier
CitationJournal of Molecular Catalysis B: Enzymatic 11(2-3): 71-79 (2000)
AbstractThe synthesis of disaccharides by galactosylation of a glucose-containing cluster has been studied using β-galactosidases from different sources. Galactosylation of the cluster could be detected by ESI-MS. After size-exclusion chromatography of the reaction mixtures and subsequent cleavage of the linker, galactopyranosyl-glucose disaccharides were obtained with yield and regioselectivity dependent on the origin of the enzyme. The possibility of using this method for the synthesis of multivalent glycoconjugates is discussed.
Description9 pages, 2 figures, 3 schemes.-- Printed version published Dec 12, 2000.
Publisher version (URL)http://dx.doi.org/10.1016/S1381-1177(00)00194-6
URIhttp://hdl.handle.net/10261/10671
DOI10.1016/S1381-1177(00)00194-6
ISSN1381-1177
Appears in Collections:(IQOG) Artículos

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