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dc.contributor.authorSatpute, Gyanesh K.es_ES
dc.contributor.authorLong, Honges_ES
dc.contributor.authorSeguí-Simarro, José Maríaes_ES
dc.contributor.authorRisueño, María Carmenes_ES
dc.contributor.authorTestillano, Pilar S.es_ES
dc.date.accessioned2009-02-12T11:54:58Z-
dc.date.available2009-02-12T11:54:58Z-
dc.date.issued2005-12-
dc.identifier.citationActa Physiologiae Plantarum 27(4):665-674(2005)es_ES
dc.identifier.issn0137-5881-
dc.identifier.urihttp://hdl.handle.net/10261/10623-
dc.description10 pages.en_US
dc.description.abstractIn this work, the cell architecture of the microspore following both gametophytic and embryogenic developmental pathways in vitro was compared with the gametophytic development in vivo in Brassica napus, at both light and electron microscopy level. The microspore reprogramming to embryogenesis involves defined changes affecting cell activities and structural organization which can be considered as markers of the microspore embryogenic pathway, but less is known about others developmental programmes followed by the microspore in vitro after both, inductive and non-inductive conditions. Low-temperature processing of the samples, cytochemical and immunocytochemical approaches to identify various cell components were performed. Differences in specific cellular features such as cellular size and shape, nuclear architecture, starch accumulation, presence of vacuoles and ribosomal population were studied to characterize sequential stages of microspore embryogenesis and other pathways occurring in vitro. The presence of abundant starch grains in a defined cytoplasmic region appeared as a specific feature of the in vitro gametophytic development, as well as of the non-induced microspores of in vitro cultures under embryogenic-inductive conditions.en_US
dc.format.extent173568 bytes-
dc.format.mimetypeapplication/pdf-
dc.language.isoenges_ES
dc.publisherPolish Academy of Scienceses_ES
dc.publisherSpringer Naturees_ES
dc.rightsopenAccessen_US
dc.subjectAmyloplastsen_US
dc.subjectIn vitro microspore cultureen_US
dc.subjectMicrospore embryogenesisen_US
dc.subjectRapeseeden_US
dc.subjectStarchen_US
dc.titleCell architecture during gametophytic and embryogenic microspore development in Brassica napus L.es_ES
dc.typeartículoes_ES
dc.identifier.doi10.1007/s11738-005-0070-y-
dc.description.peerreviewedPeer revieweden_US
dc.relation.publisherversionhttp://dx.doi.org/10.1007/s11738-005-0070-yen_US
dc.identifier.e-issn1861-1664-
dc.relation.csices_ES
dc.type.coarhttp://purl.org/coar/resource_type/c_6501es_ES
item.openairetypeartículo-
item.cerifentitytypePublications-
item.languageiso639-1en-
item.grantfulltextopen-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextWith Fulltext-
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