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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Satpute, Gyanesh K. | es_ES |
dc.contributor.author | Long, Hong | es_ES |
dc.contributor.author | Seguí-Simarro, José María | es_ES |
dc.contributor.author | Risueño, María Carmen | es_ES |
dc.contributor.author | Testillano, Pilar S. | es_ES |
dc.date.accessioned | 2009-02-12T11:54:58Z | - |
dc.date.available | 2009-02-12T11:54:58Z | - |
dc.date.issued | 2005-12 | - |
dc.identifier.citation | Acta Physiologiae Plantarum 27(4):665-674(2005) | es_ES |
dc.identifier.issn | 0137-5881 | - |
dc.identifier.uri | http://hdl.handle.net/10261/10623 | - |
dc.description | 10 pages. | en_US |
dc.description.abstract | In this work, the cell architecture of the microspore following both gametophytic and embryogenic developmental pathways in vitro was compared with the gametophytic development in vivo in Brassica napus, at both light and electron microscopy level. The microspore reprogramming to embryogenesis involves defined changes affecting cell activities and structural organization which can be considered as markers of the microspore embryogenic pathway, but less is known about others developmental programmes followed by the microspore in vitro after both, inductive and non-inductive conditions. Low-temperature processing of the samples, cytochemical and immunocytochemical approaches to identify various cell components were performed. Differences in specific cellular features such as cellular size and shape, nuclear architecture, starch accumulation, presence of vacuoles and ribosomal population were studied to characterize sequential stages of microspore embryogenesis and other pathways occurring in vitro. The presence of abundant starch grains in a defined cytoplasmic region appeared as a specific feature of the in vitro gametophytic development, as well as of the non-induced microspores of in vitro cultures under embryogenic-inductive conditions. | en_US |
dc.format.extent | 173568 bytes | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | es_ES |
dc.publisher | Polish Academy of Sciences | es_ES |
dc.publisher | Springer Nature | es_ES |
dc.rights | openAccess | en_US |
dc.subject | Amyloplasts | en_US |
dc.subject | In vitro microspore culture | en_US |
dc.subject | Microspore embryogenesis | en_US |
dc.subject | Rapeseed | en_US |
dc.subject | Starch | en_US |
dc.title | Cell architecture during gametophytic and embryogenic microspore development in Brassica napus L. | es_ES |
dc.type | artículo | es_ES |
dc.identifier.doi | 10.1007/s11738-005-0070-y | - |
dc.description.peerreviewed | Peer reviewed | en_US |
dc.relation.publisherversion | http://dx.doi.org/10.1007/s11738-005-0070-y | en_US |
dc.identifier.e-issn | 1861-1664 | - |
dc.relation.csic | Sí | es_ES |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.openairetype | artículo | - |
item.cerifentitytype | Publications | - |
item.languageiso639-1 | en | - |
item.grantfulltext | open | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | With Fulltext | - |
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ActaPhysiolPlantarum_2005_Satpute et.pdf | 3,84 MB | Adobe PDF | Visualizar/Abrir |
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