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dc.contributor.authorKalmárová, Markéta-
dc.contributor.authorKováčik, Lubomír-
dc.contributor.authorPopov, Alexey-
dc.contributor.authorTestillano, P. S.-
dc.contributor.authorSmirnov, Evgeny-
dc.identifier.citationJournal of Structural Biology 163(1):40-44(2008)en_US
dc.description5 pages, 3 figures.-- PMID: 18502146 [PubMed].-- PMCID: PMC2475583.-- Printed version published Jul 2008.en_US
dc.description.abstractRibosomal genes are organized in clusters termed Nucleolus Organizer Regions (NORs). Essential components of the RNA polymerase I transcription machinery, including Upstream Binding Factor (UBF), can be detected on some NORs during mitosis; these NORs, termed competent, are believed to be transcriptionally active during interphase. In cultured mammalian cycling cells, the number of competent NORs, and their distribution among the different chromosomes, does not vary significantly in the sequential cell cycles. In this work we investigate whether this stable state is achieved by equal distribution of competent NORs during cell division. To answer this question we first studied the state of NORs in telophase HeLa and LEP cells. In both cell lines we found a small but significant difference between the emerging daughter cells in the number of UBF-loaded NORs. To reveal the cause of this difference, we followed the fate of individual NOR using HeLa derived cell line stably expressing UBF-GFP. We demonstrated that some NORs in metaphase are “asymmetrical”, i.e. they lack the signal of competence on one of the sister chromatids. Regular presence of such NORs can account for the difference in the number of competent NORs obtained by the daughter cells emerging in mitosis.en_US
dc.description.sponsorshipThis work was supported by grants from the Wellcome Trust 075834/04/Z, Joint Project Academy of Sciences of the Czech Republic and C.S.I.C. Spain CS-ES 2007-8/16, Spanish Grant MEC-BFU2005-01094, the Ministry of Education, Youth and Sports of the Czech Republic MSM0021620806 and LC535, from the Academy of Sciences of the Czech Republic AV0Z50110509, and the Grant Agency of the Czech Republic 304/06/1662 and 304/06/1691.en_US
dc.format.extent173568 bytes-
dc.subjectNucleolus Organizer Regions (NORs)en_US
dc.subjectUpstream Binding Factor (UBF)en_US
dc.subject4D imagingen_US
dc.titleAsymmetrical distribution of the transcriptionally competent NORs in mitosisen_US
dc.description.peerreviewedPeer revieweden_US
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