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Título

Preliminary study on genetic variability of Dicrocoelium dendriticum determined by random amplified polymorphic DNA

Autor Sandoval, Hilda; Manga-González, M. Yolanda ; Campo, Raquel; García, P.; Castro, José María; Pérez de la Vega, M.
Palabras clave Dicrocoelium dendriticum
Genetic variability
Sheep
Random amplified polymorphic DNA (RAPD)
Fecha de publicación 1-mar-1999
EditorElsevier
Citación Parasitology International 48(1): 21-26 (1999)
ResumenGenetic variability of adult specimens of Dicrocoelium dendriticum has been studied using random amplified polymorphic DNA (RAPD). The worms were collected from the infected livers of different sheep from several localities in León province (NW Spain). DNA fragments were amplified by means of decamer primer oligonucleotides of arbitrary sequence. Some primers produce complex and highly variable patterns of amplified DNA in D. dendriticum. Intra- and inter-population genetic variability of adult parasites were analyzed, scoring polymorphic and monomorphic reproducible bands by means of the Jaccard similarity, and dendrograms showing genetic relationships between individuals were obtained using the FITCH method. Genetic variability seems to be high in this parasite and genetic similarity within a population (worms infecting a single animal) is similar to the average similarity between worms from different sheep. These results suggest that each sheep is infected by numerous genetically different parasites from one or more populations of infected ants.
Descripción 2 figures, 2 tables.-- PMID: 11269322 [PubMed].
Versión del editorhttp://dx.doi.org/10.1016/S1383-5769(98)00035-X
URI http://hdl.handle.net/10261/10596
DOI10.1016/S1383-5769(98)00035-X
ISSN1383-5769
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