English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/10566
COMPARTIR / IMPACTO:
Estadísticas
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Título

Eukaryotic protein production in designed storage organelles

AutorTorrent, Margarita ; Llompart, Blanca; Lasserre-Ramassamy, Sabine; Llop-Tous, Immaculada; Bastida, Miriam; Marzabal, Pau; Westerholm-Pavinen, Ann; Saloheimo, Markku; Heifetz, Peter B.; Ludevid, M. Dolors
Fecha de publicación28-ene-2009
EditorBioMed Central
CitaciónBMC Biology 7:5 (2009)
Resumen[Background] Protein bodies (PBs) are natural endoplasmic reticulum (ER) or vacuole plant-derived organelles that stably accumulate large amounts of storage proteins in seeds. The proline-rich N-terminal domain derived from the maize storage protein γ zein (Zera) is sufficient to induce PBs in non-seed tissues of Arabidopsis and tobacco. This Zera property opens up new routes for high-level accumulation of recombinant proteins by fusion of Zera with proteins of interest. In this work we extend the advantageous properties of plant seed PBs to recombinant protein production in useful non-plant eukaryotic hosts including cultured fungal, mammalian and insect cells.
[Results] Various Zera fusions with fluorescent and therapeutic proteins accumulate in induced PB-like organelles in all eukaryotic systems tested: tobacco leaves, Trichoderma reesei, several mammalian cultured cells and Sf9 insect cells. This accumulation in membranous organelles insulates both recombinant protein and host from undesirable activities of either. Recombinant protein encapsulation in these PBs facilitates stable accumulation of proteins in a protected sub-cellular compartment which results in an enhancement of protein production without affecting the viability and development of stably transformed hosts. The induced PBs also retain the high-density properties of native seed PBs which facilitate the recovery and purification of the recombinant proteins they contain.
[Conclusion] The Zera sequence provides an efficient and universal means to produce recombinant proteins by accumulation in ER-derived organelles. The remarkable cross-kingdom conservation of PB formation and their biophysical properties should have broad application in the manufacture of non-secreted recombinant proteins and suggests the existence of universal ER pathways for protein insulation.
Descripción14 pages, 5 figures.-- PMID: 19175916 [PubMed].
Versión del editorhttp://dx.doi.org/10.1186/1741-7007-7-5
URIhttp://hdl.handle.net/10261/10566
DOI10.1186/1741-7007-7-5
ISSN1741-7007
Aparece en las colecciones: (CRAG) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
Torrent_BMCBiology_2009.pdf1,11 MBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.