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Title

Functional characterization of the evolutionarily divergent fern plastocyanin

AuthorsNavarro, José A. CSIC ORCID ; Lowe, Christian E.; Amons, Reinout; Kohzuma, Takamitsu; Canters, Gerard W.; Rosa, Miguel A. de la CSIC ORCID; Ubbink, Marcellus; Hervás, Manuel CSIC ORCID
KeywordsDryopteris
Fern
Nephrolepsis
Photosystem I
Plastocyanin
Issue Date30-Jul-2004
PublisherJohn Wiley & Sons
CitationEuropean Journal of Biochemistry 271(16): 3449-3456 (2004)
AbstractPlastocyanin (Pc) is a soluble copper protein that transfers electrons from cytochrome b6f to photosystem I (PSI), two protein complexes that are localized in the thylakoid membranes in chloroplasts. The surface electrostatic potential distribution of Pc plays a key role in complex formation with the membrane-bound partners. It is practically identical for Pcs from plants and green algae, but is quite different for Pc from ferns. Here we report on a laser flash kinetic analysis of PSI reduction by Pc from various eukaryotic and prokaryotic organisms. The reaction of fern Pc with fern PSI fits a two-step kinetic model, consisting of complex formation and electron transfer, whereas other plant systems exhibit a mechanism that requires an additional intracomplex rearrangement step. The fern Pc interacts inefficiently with spinach PSI, showing no detectable complex formation. This can be explained by assuming that the unusual surface charge distribution of fern Pc impairs the interaction. Fern PSI behaves in a similar way as spinach PSI in reaction with other Pcs. The reactivity of fern Pc towards several soluble c-type cytochromes, including cytochrome f, has been analysed by flavin-photosensitized laser flash photolysis, demonstrating that the specific surface motifs for the interaction with cytochrome f are conserved in fern Pc.
Description8 pages, 7 figures.-- PMID: 15291822 [PubMed].
Publisher version (URL)http://dx.doi.org/10.1111/j.0014-2956.2004.04283.x
URIhttp://hdl.handle.net/10261/10491
DOI10.1111/j.0014-2956.2004.04283.x
ISSN0014-2956
Appears in Collections:(IBVF) Artículos

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