English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/10487
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:


IgG antibody response to ES or somatic antigens of Dicrocoelium dendriticum (Trematoda) in experimentally infected sheep

AuthorsGonzález Lanza, Camino ; Manga-González, M. Yolanda ; Campo, Raquel; Pozo Carnero, María Paz del; Sandoval, Hilda; Oleaga, Ana ; Ramajo Martín, Vicente
KeywordsFasciola hepatica
Leon province
NW Spain
Issue DateMay-2000
CitationParasitology Research 86(6): 472-479 (2000)
AbstractThe ELISA technique was used to study the kinetics of IgG antibodies against excretory–secretory (ES) and somatic (So) antigens of Dicrocoelium dendriticum in the sera of 32 lambs: 12 experimentally infected with 1,000 metacercariae, 12 with 3,000 and 8 controls. Both antigen types were used at a 2 μg/ml concentration. Dilutions of sera and the anti-sheep IgG peroxidase conjugate were: 1:200 and 1:6,000, respectively. Optical density values for each type of antigen in the two infected groups were higher than the cut-off point from day 30 post infection (p.i.), showing positive infection. Maximum antibody levels were observed 60 days p.i. and remained high until the experiment ended 180 days p.i. This pattern was similar for both ES and So antigens, although with slightly lower figures in the latter. Antibody kinetics were very similar for each of the two doses, except on day 30 p.i. No correlation between the antibody level and parasite burden could be established.
Description8 pages, 2 figures, 2 tables.-- PMID: 10894473 [PubMed].
Publisher version (URL)http://dx.doi.org/10.1007/s004360050696
Appears in Collections:(IRNASA) Artículos
(IGM) Artículos
Files in This Item:
There are no files associated with this item.
Show full item record
Review this work

Related articles:

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.