Isotopic composition of carbon and nitrogen of suprabenthic fauna in the NW Balearic Islands (western Mediterranean)

Abstract

Stable isotope (δ13C and δ15N) analyses were performed on suprabenthic fauna collected in the western Mediterranean (NW Balearic Islands), at depths ranging between 350 and 780 m. Samples were collected seasonally at bi-monthly intervals during six cruises performed between August 2003 and June 2004, using a Macer-GIROQ suprabenthic sledge (0.5 mm mesh size). Twenty-four separate species (5 mysids, 12 amphipods, 2 cumaceans, 2 isopods, 1 euphausiid, 1 decapod and 1 fish) and bulk copepods were analyzed on a seasonal basis for stable carbon and nitrogen isotopes. Stable nitrogen isotope ratios (δ15N) ranged from 2.3‰ (the amphipod Lepechinella manco in September 2003) to 13.0‰ (the amphipod Rhachotropis caeca in August 2003). δ13C values ranged from - 24.2 (the cumacean Campylaspis sulcata in June 2004) to - 16.1 (the amphipod Bruzelia typica in November 2006). Both δ13C and δ15N values suggest that there are three trophic levels within the suprabenthic community. However, considering the bathymetric range of the species, the results suggest that the deepest assemblage supported only two trophic levels. The stable isotope ratios of suprabenthic fauna displayed a continuum of values and confirmed a wide spectrum of feeding types (from filter-feeders to predators). In general, and in spite of the poor knowledge about diets available for most suprabenthic species, higher δ15N were found for carnivorous amphipods (e.g. Rhachotropis spp., Nicippe tumida) consuming copepods. Low overlap for δ13C and δ15N values was observed, though δ15N values where less variable than δ13C, which suggests high resource partitioning in this assemblage. Seasonal variations in isotopic composition for both δ13C and δ15N were low (less than 1‰ and 3‰, respectively) and variable depending on species. Low correlations between δ13C and δ15N of suprabenthic fauna were found for all periods studied, though increasing from February 2004 to June 2004 (after the main peak of primary production in surface). C:N ratio (indicator of lipid content) showed higher values in summer than in winter. This suggests that lipid content may explain the seasonal patterns of δ13C variability and, due to the increase of storage products in phytoplankton and zooplankton, it possibly indicates the peak of primary production at the surface. © 2007 Elsevier B.V. All rights reserved