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Detailed architecture of a DNA translocating machine: The high-resolution structure of the bacteriophage φ29 connector particle

AuthorsGuasch, Alicia ; Pous, Joan ; Ibarra, Borja; Gomis-Rüth, F. Xavier ; Valpuesta, José M.; Sousa, Natalia; Carrascosa, José L.; Coll, Miquel
KeywordsConnector particle
DNA packaging machinery
X-ray difraction
Bacteriophage phi29
Issue Date2002
CitationJournal of Molecular Biology 315(4): 663-676 (2002)
AbstractThe three-dimensional crystal structure of the bacteriophage φ29 connector has been solved and refined to 2.1 Å resolution. This 422 kDa oligomeric protein connects the head of the phage to its tail and translocates the DNA into the prohead during packaging. Each monomer has an elongated shape and is composed of a central, mainly α-helical domain that includes a three-helix bundle, a distal α/β domain and a proximal six-stranded SH3-like domain. The protomers assemble into a 12-mer, propeller-like, super-structure with a 35 Å wide central channel. The surface of the channel is mainly electronegative, but it includes two lysine rings 20 Å apart. On the external surface of the particle a hydrophobic belt extends to the concave area below the SH3-like domain, which forms a crown that retains the particle in the head. The lipophilic belt contacts the non-matching symmetry vertex of the capsid and forms a bearing for the connector rotation. The structure suggests a translocation mechanism in which the longitudinal displacement of the DNA along its axis is coupled to connector spinning. © 2002 Academic Press.
Identifiersdoi: 10.1006/jmbi.2001.5278
issn: 0022-2836
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