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Title

Quality assessment of two ecologically important exosystems in the south coast of Portugal - Oxidative stress biomarkers and proteomic analysis in Scrobicularia Plana

AuthorsGomes, Tania; Pereira, C.; Rodríguez, Antonio ; Macías, Diego ; González-Rey, María; Blasco, Julián ; Bebianno, Maria João
KeywordsEstudios proteómicos
Proteomic analysis
Biomarker approach
Aproximación biomarcadores
Issue DateJan-2012
CitationXVI Seminario Ibérico de Química Marina (2012)
AbstractAquatic environments in the south of Portugal support several high biodiversity ecological ecosystems that have their environmental situation critically dependent on the contributions of mining activities, increase of population densities, agricultural and industrial activities. The evaluation of contaminant impacts on marine organisms and consequent quality of coastal zones is crucial and requires efficient analytical methodologies combined with new management tools. In order to assess the environmental risk of contamination in these ecosystems data obtained from several Interreg IIIA framework projects (RED CONTAMAR, PROTEOBIOMAR and RISE) was gathered and analysed. Accordingly, the environmental quality of two important aquatic ecosystems of south of Portugal, the Ria Formosa Lagoon and the Guadiana Estuary, was assessed carrying out an integrated approach combining conventional oxidative stress biomarkers (SOD, CAT, GPX, and LPO) with proteomic analysis. Clams were collected in the Ria Formosa and in the Guadiana Estuary, in two winter periods (2007 and 2010). The biomarker approach indicated site and temporal related differences. As for proteomic analysis, several differences were detected in protein expression signatures between sites and winter periods. The highest changes in protein expression profiles (PEPs) corresponded to clams from the Guadiana Estuary possibly reflecting differences between contaminant loads as well as their interactions in complex mixtures. The comparison of PEPs through time could lead to the development of new biomarkers to be used in environmental quality assessment and its integration with biomarker responses in S. plana can be a useful tool for marine pollution monitoring in these ecosystems.
DescriptionTrabajo presentado en el XVI Seminario Ibérico de Química Marina, celebrado en Cádiz del 24 al 26 de enero de 2012.
URIhttp://hdl.handle.net/10261/104223
Appears in Collections:(ICMAN) Comunicaciones congresos
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