Descripción histomorfológica del tejido esquelético de juveniles de dorada, Sparus aurata, con malformaciones vertebrales y operculares

Abstract

A comparative study of the histological morphology of normal and deformed vertebrae and operculum of Sparus aurata juveniles by means of histological (Haematoxylin/Eosin), hist ochemical (Alcian blue-AB, Picrosirius red–PR, Tart rate-resistant acid phosphatase-TRAP) and inmunohistochemical (Osteocal cin-Oc, Caspase-3 and PCNA) approaches was performe d. PR staining showed some differences in bone matrix composition between normal and deformed vertebra and operculum. These results were corroborated under polarization optic microscope. P CNA as a marker of cell proliferation also presente d differences in its distribution between normal and deformed vertebral bodies and operculum, with a marked increase in pos itive cells in the growth zone of the endplates from deformed vertebra. Howev er, Caspase-3 as a marker of cell apoptosis did not show such differences. TRAP activity detected in trabecular bone from heal thy sea bream specimens was lacked in deformed ones , suggesting that normal endochondral ossification was restrained. Finally, Oc changed its bone matrix distribution appearing i n notochordal tissue of deformed vertebra.