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Two isoforms of vasa maternal factor in Solea senegalensis: biotechnological applications

AuthorsPacchiarini, Tiziana ; Cabrita, Elsa; Cross, Ismael; Ortiz-Delgado, Juan B. ; Leite, Ricardo B.; Gavaia, Paulo J.; Herráez, M. P.; Rebordinos, Laureana; Sarasquete, Carmen
Issue DateApr-2011
CitationV Workshop The cultivation of the Soles (2011)
AbstractIn the last decade vasa homologs have been cloned in several teleosts and their expression patterns during oogenesis, spermatogenesis and development have been analyzed. These previous studies show that the vasa gene is a germ cells molecular marker with a high biotechnological potential for identification and manipulation of germ cells in surrogate broodstocks. In teleosts different expression patterns were observed between males and females (Kobayashi et al., 2000) and during embryonic and larval development (Krøvel and Olsen, 2004) for vasa isoforms. In this study two isoforms of vasa gene in S. senegalensis were identified, characterized and the expression pattern was described. The Senegalese sole vasa cDNA 1 (Ssvasa1) was 2374bp while the Senegalese sole vasa cDNA 2 (Ssvasa2) was 1993bp. Ssvasa isoforms showed high homology with others vasa cDNAs when submitted at Blast analysis. Resulting amino acidic sequences of Ssvasa isoform were clustered in the VASA subfamily when compared with others DEAD box protein by neighbourjoining analysis. The RT-PCR and Q-PCR analysis performed in different tissues of adult fishes showed that Ssvasa gene expression was restricted to ovary and testis. Our results showed that the Ssvasa1 RNA is maternally provided since expression was only detected until mid-blastula transition stage. Expression patterns of Ssvasa1 and Ssvasa2 were different during embryonic and larval development, similar with the observed in zebrafish (Krøvel and Olsen, 2004). Our results demonstrate that Ssvasa1 RNA could be used as a molecular marker for testicular germ cells while Ssvasa2 is more appropriate for the study of primordial germ cells during embryonic and larval development in Senegalese sole.
DescriptionTrabajo presentado en el V Workshop The cultivation of the Soles, celebrado en Faro (Portugal) del 5 al 7 de abril de 2011.
Appears in Collections:(ICMAN) Comunicaciones congresos
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