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Título: | Relation between pathologic findings and cellular immune responses in sheep with naturally acquired paratuberculosis |
Autor: | Pérez Pérez, Valentín CSIC ORCID ; Tellechea, J.; Corpa, J. M.; Gutiérrez, M.; García Marín, Juan Francisco CSIC | Palabras clave: | Classification Absorbed Elisa Interferon-Gamma Mycobacterium-Paratuberculosis Spectrum Agar-Gel Immunodiffusion Diagnosis Infection Ruminant Paratuberculosis Tuberculosis Johnes Disease |
Fecha de publicación: | 1999 | Editor: | American Veterinary Medical Association | Citación: | American Journal of Veterinary Research 60: 123-127 (1999) | Resumen: | Objective-To investigate the relation between lesions and cellular immune response-based tests in sheep with naturally acquired paratuberculosis and to evaluate efficacy of an interferon-gamma (IFN-gamma) assay and the intradermal skin test. Animals-110 and 88 adult sheep were used to evaluate the IFN-gamma assay and skin test, respectively. Procedure-Before euthanasia, heparinized blood was collected and incubated with avian purified protein derivative (PPD). Interferon-gamma production was assessed, using a commercial enzyme immunoassay. Skin testing in the caudal fold also was performed, using avian PPD. Sensitivity and specificity of these techniques were evaluated, using histopathologic findings as reference. Results-Of the sheep examined, 61 (55.4%) had lesions that were classified in 5 categories, according to location, extension, cellular types, and presence of acid-fast bacilli. Regarding cellular immunity lest results, 55.4% of sheep with and 6.2% without pathologic changes had positive results of the IFN-gamma assay. Skin test sensitivity (55.6%) and specificity (100%) were slightly greater. Conclusion-Close association between pathologic findings and cellular immune response was apparent. Most sheep with focal (tuberculoid) and some with diffuse lesions (borderline tuberculoid), all with scant or no acid-fast bacilli in the intestine, had positive results of the IFN-gamma, assay and skin test. Most sheep with diffuse lesions and high numbers of bacilli (borderline lepromatous) were test negative. Clinical Relevance-Cellular immune response-based and serologic tests are useful tools for seeking evidence of paratuberculosis. Each technique detects different types of immune-reacting sheep, and their simultaneous use may detect most infected animals. | Descripción: | 5 páginas. | Versión del editor: | https://www.avma.org/Pages/home.aspx | URI: | http://hdl.handle.net/10261/104136 | ISSN: | 0002-9645 |
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