English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/104062
Compartir / Impacto:
Estadísticas
Add this article to your Mendeley library MendeleyBASE
Citado 10 veces en Web of Knowledge®  |  Ver citas en Google académico
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar otros formatos: Exportar EndNote (RIS)Exportar EndNote (RIS)Exportar EndNote (RIS)
Título

Influence of surface features of hydroxyapatite on the adsorption of proteins relevant to bone regeneration.

Autor Fernández-Montes, Belén ; San Román, Julio ; Rodríguez-Lorenzo, Luis M.
Palabras clave Fibronectin adsorption
Fibrinogen adsorption
hydroxyapatite
Raman
Protein binding
Fecha de publicación 2013
EditorJohn Wiley & Sons
Citación Journal of Biomedical Materials Research - Part A 101A: 2332-2339 (2013)
ResumenProtein-surface interaction may determine the success or failure of an implanted device. Not much attention have been paid to the specific surface parametes of hydroxyapatite (OHAp) that modulates and determines the formation and potential activity of the layer of proteins that is first formed when the material get in contact with the host tissue. the influence of specific surface area (SSA), crystallite size (CS) and particle size (PS) of OHAp on the adsorption of proteins relevant for bone regeneration is evaluated in this article. OHAp have been prepared by a wet chemical reaction of Ca(OH)(2) with H(3) PO(4) . One set of reactions included poly acrylic acid in the reactant solution to modify the properties of the powder. Fibrinogen (Fg) Fraction I, type I: from Human plasma, (67% Protein), and Fibronectin (Fn) from Human plasma were selected to perform the adsorption experiments. The analysis of protein adsorption was carried out by UV/Vis spectrometry. A lower SSA and a different aspect ratio are obtained when the acrylic acid is included in the reaction badge. The deconvolution of the amide I band on the Raman spectra of free and adsorbed proteins reveals that the interaction apatite-protein happens through the carboxylate groups of the proteins. The combined analysis of CS, SSA and PS should be considered on the design of OHAp materials intended to interact with proteins.
URI http://hdl.handle.net/10261/104062
DOI10.1002/jbm.a.34528
Identificadoresdoi: 10.1002/jbm.a.34528
issn: 1549-3296
e-issn: 1552-4965
Aparece en las colecciones: (ICTP) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
accesoRestringido.pdf15,38 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 



NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.