English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/10393
Título

Vaccination of mice and sheep with Fh12 FABP from Fasciola hepatica using the new adjuvant/immunomodulator system ADAD

AutorMartínez-Fernández, Antonio R.; Nogal-Ruiz, Juan José; López-Abán, J.; Ramajo Martín, Vicente ; Oleaga, Ana ; Manga-González, M. Yolanda ; Hillyer, G. V.; Muro, Antonio
Palabras claveFasciola hepatica
Vaccine
FABP
ADAD system
Fecha de publicación2004
EditorElsevier
CitaciónVeterinary Parasitology 126(3): 287-298 (2004)
ResumenWe evaluate the ability of a Fasciola hepatica FABP native antigen (Fh12) with a new vaccination system called ADAD to protect mice and sheep against an experimental F. hepatica infection. The vaccination protocol consists of a set of two injections. The first injection contains a micelle in which two components are included, saponin from Quillaja saponaria (Qs) and/or Anapsos (A) a Polypodium leucotomos hydroalcoholic extract, both emulsified in a non-mineral oil (Montanide) in a water/oil emulsion (30/70). This is subcutaneously injected to achieve the “adaptation” of the immune system to subsequent stimuli. The second injection contains in addition the Fh12 antigen. Two different experiments were carried out using two mouse strains (BALB/c and CD-1). Mice vaccinated with Qs + A + Fh12 presented a survival rate of 40%, when compared with control groups. Furthermore, we evaluated the efficiency of the vaccination in sheep against an experimental F. hepatica challenge. The vaccinated sheep presented lower fluke recovery (24.5%), number of eggs in bile fluid (58.1%) and faeces (40.3%) than control groups. The recovered flukes were shorter (32.7%), immature (34.0%) and with lower body mass (31.6%) than non-complete vaccinated sheep. Thus, the new ADAD system could be a good alternative for future vaccination experiments against fasciolosis.
Descripción12 pages, 4 figures.-- PMID: 15567592 [PubMed].-- Available online 8 Oct 2004.
Versión del editorhttp://dx.doi.org/10.1016/j.vetpar.2004.07.023
URIhttp://hdl.handle.net/10261/10393
DOI10.1016/j.vetpar.2004.07.023
ISSN0304-4017
Aparece en las colecciones: (IRNASA) Artículos
(IGM) Artículos
Ficheros en este ítem:
No hay ficheros asociados a este ítem.
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.