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European seabass (Dicentrarchus labrax) sperm modulation to improve antioxidant system for cryopreservation

AuthorsMartínez-Páramo, S.; Cerqueira, Marco; Diogo, P.; Dinis, M. T.; Sarasquete, Carmen ; Cabrita, Elsa
Issue DateSep-2011
Citation3rd International Workshop on Biology of Fish Gametes: final programme and books of abstracts: 158-159 (2011)
AbstractOxidative stress, as a result of the imbalance between the reactive oxygen species (ROS) and the sperm antioxidant system, promotes sperm morphological and physiological damage, since all cellular components (lipids, proteins and nucleic acids) are potential targets for ROS. Although antioxidant defense system is active in semen, its activity is limited as the amount of spermatozoa cytoplasm is low (Shiva et al. 2011). Moreover, dilution in the extender media for cryopreservation reduces the seminal plasma constituents being sperm more vulnerable to oxidative stress. Oxidative damage can be reduced by sperm modulation through breeders’ diet (Cerolini et al. 2006) or by antioxidant addition in the freezing media (Cabrita et al. 2011). Thus, in the present work, these two ways of modulation were tested to improve the antioxidant system of seabass sperm by enrichment of HUFAs, vitamin E and Selenium (Se), with the aim to reduce cryodamage.
DescriptionTrabajo presentado en el 3rd International Workshop on Biology of Fish Gametes, celebrado en Budapest del 7 al 9 de septiembre de 2011.
Identifiersisbn: 978-963-88019-7-5
Appears in Collections:(ICMAN) Libros y partes de libros
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