English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/103850
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Exportar a otros formatos:

Title

Toxicity of copper oxide nanoparticles in the blue mussel, Mytilus edulis: A redox proteomic investigation

AuthorsHu, Wentao; Culloty, Sarah; Darmody, Grainne; Lynch, Sharon; Davenport, John; Ramírez-García, Sonia; Dawson, Kenneth A.; Lynch, Iseult; Blasco, Julián ; Sheehan, David
KeywordsNanoparticles
Protein carbonyl
Oxidative stress
Copper
Protein thiols
Issue DateAug-2014
PublisherElsevier
CitationChemosphere 108: 289-299 (2014)
AbstractRelatively little is known about the fate and effects of nanomaterials even in relatively simple organisms such as Mytilus edulis. Here, copper oxide nanoparticles (CuO NP) are shown to induce dose-dependent toxic effects at the biochemical, physiological and tissue levels in the blue mussel. Stable CuO NP suspensions were sized by differential light scattering and nanoparticle tracking analysis to yield average particle diameters of approximately 100. nm. These were administered to M. edulis, at doses of 400, 700 and 1000. ppb. Ingested copper was predominantly located in the gill tissue with small amounts in digestive gland. Fifteen coomassie-stained spots were excised from two dimensional gel electrophoresis separations of gill tissue extacts and identified by peptide mass fingerprinting. These contained six unique proteins (alpha- and beta-tubulin, actin, tropomyosin, triosephosphate isomerase and Cu-Zn superoxide dismutase). Of these, two spots (actin and triosephosphate isomerase) showed decreased protein thiols while three (alpha-tubulin, tropomyosin and Cu-Zn superoxide dismutase) showed increased carbonylation which is indicative of protein oxidation of cytoskeleton and enzymes in response to CuO NP. The neutral red retention time (NRRT) assay revealed toxicity due to the CuO NPs which was comparable with toxic metal oxide nanoparticles such as chromium and cobalt. In contrast, non-toxic titanium and gold metal oxide nanoparticles gave no NRRT effects at similar NP concentrations. Histology revealed deposition of pigmented brown cells in response to CuO NP, located predominantly along the mantle and gill margin but also lining digestive tubules and some of the sinuses and distributed throughout the connective tissue and in the adductor muscle. © 2014 Elsevier Ltd.
Publisher version (URL)http://doi.org/10.1016/j.chemosphere.2014.01.054
URIhttp://hdl.handle.net/10261/103850
DOIhttp://dx.doi.org/10.1016/j.chemosphere.2014.01.054
Identifiersdoi: 10.1016/j.chemosphere.2014.01.054
issn: 1879-1298
Appears in Collections:(ICMAN) Artículos
Files in This Item:
File Description SizeFormat 
accesoRestringido.pdf15,38 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.