English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/10384
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Exportar a otros formatos:

Title

Role of septins and the exocyst complex in the function of hydrolytic enzymes responsible for fission yeast cell separation

AuthorsMartín-Cuadrado, Ana Belén; Rey Iglesias, Francisco del ; Vázquez de Aldana, Carlos R.
Issue Date3-Aug-2005
PublisherAmerican Society for Cell Biology
CitationMolecular Biology of the Cell 16(10): 4867-4881 (2005)
AbstractCell separation in Schizosaccharomyces pombe is achieved by the concerted action of the Eng1 endo-β-1,3-glucanase and the Agn1 endo-α-1,3-glucanase, which are transported to the septum and localize to a ringlike structure that surrounds the septum. The requirements for the correct localization of both hydrolases as a ring were analyzed using green fluorescent protein fusion proteins. Targeting to the septum required a functional exocyst, because both proteins failed to localize correctly in sec8-1 or exo70Δ mutants, suggesting that Agn1 and Eng1 might be two of the cargo proteins present in the vesicles that accumulate in exocyst mutants. Septins and Mid2 were also required for correct formation of a ring. In their absence, Eng1 and Agn1 were found in a disklike structure that spanned the septum, rather than in a ring. Even though septin and mid2Δ mutants have a cell separation defect, the septum and the distribution of linear β-1,3-glucans were normal in these cells, suggesting that mislocalization of Eng1 and Agn1 might be the reason underlying the failure to separate efficiently. Thus, one of the functions of the septin ring would be to act as a positional marker for the localization of hydrolytic proteins to the medial region.
Description15 pages, 11 figures.-- et al.
Publisher version (URL)http://dx.doi.org/10.1091/mbc.E04-12-1114
URIhttp://hdl.handle.net/10261/10384
DOI10.1091/mbc.E04-12-1114
ISSN1059-1524
Appears in Collections:(IMB) Artículos
Files in This Item:
File Description SizeFormat 
39722_Septinas AnaB.pdf979,14 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.