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Title:	The Cdc14p phosphatase affects late cell-cycle events and morphogenesis in Candida albicans
Authors:	González-Novo, Alberto ; Sánchez-Pérez, Miguel; Vázquez de Aldana, Carlos R. ; Jiménez, Javier; Correa-Bordes, Jaime
Keywords:	Cytokinesis Cell separation Mitotic exit ACE2 HGC1
Issue Date:	28-Feb-2006
Publisher:	Company of Biologists
Citation:	Journal of Cell Science 119(6): 1130-1143 (2006)
Abstract:	We have characterized the CDC14 gene, which encodes a dual-specificity protein phosphatase in Candida albicans, and demonstrated that its deletion results in defects in cell separation, mitotic exit and morphogenesis. The C. albicans cdc14Δ mutants formed large aggregates of cells that resembled those found in ace2-null strains. In cdc14Δ cells, expression of Ace2p target genes was reduced and Ace2p did not accumulate specifically in daughter nuclei. Taken together, these results imply that Cdc14p is required for the activation and daughter-specific nuclear accumulation of Ace2p. Consistent with a role in cell separation, Cdc14p was targeted to the septum region during the M-G1 transition in yeast-form cells. Interestingly, hypha-inducing signals abolished the translocation of Cdc14p to the division plate, and this regulation depended on the cyclin Hgc1p, since hgc1Δ mutants were able to accumulate Cdc14p in the septum region of the germ tubes. In addition to its role in cytokinesis, Cdc14p regulated mitotic exit, since synchronous cultures of cdc14Δ cells exhibited a severe delay in the destruction of the mitotic cyclin Clb2p. Finally, deletion of CDC14 resulted in decreased invasion of solid agar medium and impaired true hyphal growth.
Description:	14 pages, 10 figures.-- et al.
Publisher version (URL):	http://dx.doi.org/10.1242/jcs.02820
URI:	http://hdl.handle.net/10261/10381
DOI:	10.1242/jcs.02820
ISSN:	0021-9533
Appears in Collections:	(IMB) Artículos