English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/10376
Compartir / Impacto:
Estadísticas
Add this article to your Mendeley library MendeleyBASE
Citado 26 veces en Web of Knowledge®  |  Pub MebCentral Ver citas en PubMed Central  |  Ver citas en Google académico
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar otros formatos: Exportar EndNote (RIS)Exportar bibText (RIS)Exportar csv (RIS)
Título

Characterization of the endo-β-1,3-glucanase activity of S. cerevisiae Eng2 and other members of the GH81 family

Autor Martín-Cuadrado, Ana Belén; Esteban, Pedro Felipe; Encinar, Javier; Medina-Redondo, María de; Rey Iglesias, Francisco del; Vázquez de Aldana, Carlos R.
Palabras clave Endo-β-1,3-glucanase
Glycoside hydrolase
Catalytic domain
Active site
Fecha de publicación abr-2008
EditorElsevier
Citación Fungal Genetics and Biology 45(4): 542-553 (2008)
ResumenThe GH81 family includes proteins with endo-β-1,3-glucanase widely distributed in yeast and fungi, which are also present in plants and bacteria. We have studied the activity of the Saccharomyces cerevisiae ScEng2 and the Schizosaccharomyces pombe SpEng1 and SpEng2 proteins. All three proteins exclusively hydrolyzed linear β-1,3-glucan chains. Laminari-oligosaccharide degradation revealed that the minimum substrate length that the three endoglucanases were able to efficiently degrade was a molecule with at least 5 glucose residues, suggesting that the active site of the enzymes recognized five glucose units. Prediction of the secondary structure of ScEng2 and comparison with proteins of known structure allowed the identification of a 404-amino acid region with a structure similar to the Clostridium thermocellum endoglucanase CelA. This fragment showed similar enzymatic characteristics to those of the complete protein, suggesting that it contains the catalytic domain of this family of proteins. Within this domain, four conserved Asp and Glu residues (D518, D588, E609, and E613) are necessary for enzymatic activity.
Descripción 12 pages, 6 figures.-- et al.
Versión del editorhttp://dx.doi.org/10.1016/j.fgb.2007.09.001
URI http://hdl.handle.net/10261/10376
DOI10.1016/j.fgb.2007.09.001
ISSN1087-1845
Aparece en las colecciones: (IMB) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
accesoRestringido.pdf15,38 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 



NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.