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Composición de la comunidad procariota involucrada en la producción de nitrógeno en sedimentos de la bahía Mejillones

Other TitlesProkaryotic community composition involved production of nitrogen in sediments of Mejillones Bay
AuthorsMoraga, Rubén; Galán, Alexander; Rosselló-Mora, Ramón ; Araya, Rubén; Valdés, Jorge L.
KeywordsMarine sediments
Production of denitrification
Issue Date2014
PublisherUniversidad de Valparaíso
CitationRevista de Biologia Marina y Oceanografia 49(2): 225-241 (2014)
AbstractConventional denitrification and anaerobic ammonium oxidation (anammox) contributes to nitrogen loss in oxygen-deficient systems, thereby influencing many aspects of ecosystem function and global biogeochemistry. Mejillones Bay, northern Chile, presents ideal conditions to study nitrogen removal processes, because it is inserted in a coastal upwelling system, its sediments have anoxia and hypoxia conditions and under the influence of the Oxygen Minimum Zone (OMZ), unknown processes that occur there and what are the microbial communities responsible for their removal. Microbial communities associated with coastal sediments of Mejillones Bay were studied by denaturing gel electrophoresis (DGGE) and fluorescence in situ hybridization (FISH), by incubation experiments with 15N isotope tracers were studied nitrogen loss processes operating in these sediments. DGGE analysis showed high bacterial diversity, certain redundant phylotypes and differences in community structure given by the depth; this reflects the microbial community adaptations to environmental conditions. A large fraction (up to 70%) of DAPI-stained cells hybridized with the bacterial probes. Nearly 52-90% of the cell could be further identified to know phyla. Members of the Cytophaga-Flavobacterium cluster were most abundant in the sediments (13-26%), followed by Proteobacteria. Isotopic tracer experiments for the sediments studied indicated that nitrogen loss processes that predominated were performed by denitrifying communities (43.31-111.20 μM d-1) was not possible to detect anammox in the area and not anammox bacteria were detected.
Identifierse-issn: 0718-1957
issn: 0717-3326
Appears in Collections:(IMEDEA) Artículos
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