English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/10349
Compartir / Impacto:
Estadísticas
Add this article to your Mendeley library MendeleyBASE
Citado 27 veces en Web of Knowledge®  |  Pub MebCentral Ver citas en PubMed Central  |  Ver citas en Google académico
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar otros formatos: Exportar EndNote (RIS)Exportar bibText (RIS)Exportar csv (RIS)
Título

A proteomic approach to the identification of salivary proteins from the argasid ticks Ornithodoros moubata and Ornithodoros erraticus

AutorOleaga, Ana ; Escudero Población, Andrés; Camafeita, Emilio; Pérez Sánchez, Ricardo
Palabras claveOrnithodoros
Salivary gland extract
Saliva
Proteome
Mass spectrometry
Fecha de publicación13-jul-2007
EditorElsevier
CitaciónInsect Biochemistry and Molecular Biology 37(11): 1149-1159 (2007)
ResumenThe saliva of ticks contains anti-haemostatic, anti-inflammatory and immunomodulatory molecules that allow these parasites to obtain a blood meal from the host and help tick-borne pathogens to infect the vertebrate host more efficiently. This makes the salivary molecules attractive targets to control ticks and tick-borne pathogens. Although Ornithodoros moubata and O. erraticus are important argasid ticks that transmit severe diseases, to date only a few of their salivary proteins have been identified. Here we report our initial studies using proteomic approaches to characterize the protein profiles of salivary gland extracts (SGE) from these two argasids. The present work describes the proteome of the SGEs of both tick species, their antigenic spots, and the identification of several of their proteins. The whole number of identifications was low despite the good general quality of the peptide mass maps obtained. In the O. moubata SGE, 18 isoforms of a protein similar to O. savignyi TSGP1 were identified. In the O. erraticus SGE we identified 6 novel proteins similar to unknown secreted protein DS-1 precursor, NADPH dehydrogenase subunit 5, proteasome alpha subunit, ATP synthase F0 subunit 6, lipocalin and alpha tubulin. Finally, the current drawbacks of proteomics when applied to the identification of acarine peptides and proteins are discussed.
Descripción11 pages, 3 figures.-- PMID: 17916501 [PubMed].-- Printed version published Nov 2007.
Versión del editorhttp://dx.doi.org/10.1016/j.ibmb.2007.07.003
URIhttp://hdl.handle.net/10261/10349
DOI10.1016/j.ibmb.2007.07.003
ISSN0965-1748
Aparece en las colecciones: (IRNASA) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
92209_IB-D-07-00097_final_manuscript.pdf2,1 MBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 



NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.