English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/10344
Compartir / Impacto:
Estadísticas
Add this article to your Mendeley library MendeleyBASE
Citado 7 veces en Web of Knowledge®  |  Pub MebCentral Ver citas en PubMed Central  |  Ver citas en Google académico
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Título

Thermal unfolding of plastocyanin from the mesophilic cyanobacterium Synechocystis sp. PCC 6803 and comparison with its thermophilic counterpart from Phormidium laminosum

AutorFeio, Maria J.; Díaz-Quintana, Antonio; Navarro, José A. ; Rosa, Miguel A. de la
Fecha de publicación23-mar-2006
EditorAmerican Chemical Society
CitaciónBiochemistry 45(15): 4900-4906 (2006)
ResumenThe thermal unfolding of plastocyanin from the mesophilic cyanobacterium Synechocystis is described herein, and the results are compared with those obtained for the homologous thermophilic protein from Phormidium laminosum. The thermal unfolding is irreversible under all the conditions that were investigated. Plastocyanin from the thermophilic organism, both in the native state and in the apoprotein form, proved to be more thermostable than its mesophilic counterpart under all experimental conditions. Synechocystis reduced plastocyanin has been shown to be more stable than the oxidized species, both with respect to the required temperature for protein unfolding and with respect to the kinetics of the process. This behavior contrasts with that observed for Phormidium plastocyanin, in which the oxidized form is the more stable one. The unfolding pH dependence and kinetic studies indicate that around physiological pH, the most kinetically stable form is also the one more resistant to temperature variations, suggesting a close compromise between function and stability. Molecular dynamics simulations suggest that Phormidium and Synechocystis plastocyanins follow different unfolding pathways that affect different protein areas and which could be responsible for the observed dissimilar thermal resistance.
Descripción7 pages, 5 figures.-- PMID: 16605257 [PubMed].-- Printed version published Apr 18, 2006.
Versión del editorhttp://dx.doi.org/10.1021/bi052312q
URIhttp://hdl.handle.net/10261/10344
DOI10.1021/bi052312q
ISSN0006-2960
Aparece en las colecciones: (IBVF) Artículos
Ficheros en este ítem:
No hay ficheros asociados a este ítem.
Mostrar el registro completo
 



NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.