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Immunoaffinity, capillary electrophoresis, and statistics for studying intact alpha 1-acid glycoprotein isoforms as an atherothrombosis biomarker

AutorPuerta, Angel de la ; Martín-Álvarez, Pedro J. ; Ongay, Sara ; Diez-Masa, Jose Carlos; Frutos, Mercedes de
Palabras claveBiomarker
Vascular disease
Statistical analysis
Capillary electrophoresis
Isoform separation
Glycoprotein
Immunochromatography
Affinity chromatography
Purification
Fecha de publicación2013
EditorSpringer
CitaciónClinical Applications of Capillary Electrophoresis: 215-230 (2013)
SerieMethods in Molecular Biology
919
ResumenVariations in the amino acid sequence, glycosylation, and/or other posttranslational modifications in glycoproteins give rise to different molecules of the glycoprotein called forms. Qualitative and/or quantitative alterations in these forms are related to pathophysiological situations in the individuals. In this study, a methodology to analyze these differences in forms of the alpha 1-acid glycoprotein (AGP) between healthy individuals and patients with two different vascular diseases is detailed. The whole methodology includes a sample preparation method based on immunochromatography, a capillary electrophoresis method for separation of AGP peaks (isoforms), and statistical methods (Linear Discriminant Analysis) for sample classification. As a result, it is shown that the methodology proposed allows studying the role of AGP isoforms as potential vascular disease biomarkers.
DescripciónCapítulo 20.
URIhttp://hdl.handle.net/10261/103428
DOI10.1007/978-1-62703-029-8_20
ISBN978-1-62703-028-1
Identificadoresdoi: 10.1007/978-1-62703-029-8_20
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