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dc.contributor.authorRuiz-Masó, José A.-
dc.contributor.authorLurz, Rudi-
dc.contributor.authorEspinosa, Manuel-
dc.contributor.authorSolar, Gloria del-
dc.identifier.citationNucleic Acids Research 2007, 35 (4) 1230–1244es_ES
dc.description15 p.-7 fig.-1 tab.es_ES
dc.description.abstractPlasmids replicating by the rolling circle mode usually possess a single site for binding of the initiator protein at the origin of replication. The origin of pMV158 is different in that it possesses two distant binding regions for the initiator RepB. One region was located close to the site where RepB introduces the replication-initiating nick, within the nic locus; the other, the bind locus, is 84 bp downstream from the nick site. Binding of RepB to the bind locus was of higher affinity and stability than to the nic locus. Contacts of RepB with the bind and nic loci were determined through high-resolution footprinting. Upon binding of RepB, the DNA of the bind locus follows a winding path in its contact with the protein, resulting in local distortion and bending of the double-helix. On supercoiled DNA, simultaneous interaction of RepB with both loci favoured extrusion of the hairpin structure harbouring the nick site while causing a strong DNA distortion around the bind locus. This suggests interplay between the two RepB binding sites, which could facilitate loading of the initiator protein to the nic locus and the acquisition of the appropriate configuration of the supercoiled DNA substrate.es_ES
dc.description.sponsorshipJ. A. R-M. was a recipient of a FPI fellowship from the Ministerio de Educación y Ciencia. Research financed by Ministerio de Educación y Ciencia (grant BFU2004-00687/BMC to G.d.S. and Acción Especial BMC2002-11562-E to M.E.).es_ES
dc.publisherOxford University Presses_ES
dc.relation.isversionofPublisher's versiones_ES
dc.titleInteractions between the RepB initiator protein of plasmid pMV158 and two distant DNA regions within the origin of replicationes_ES
dc.description.peerreviewedPeer reviewedes_ES
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