English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/10328
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Title

Carbohydrate profiling and protein identification of tegumental and excreted/secreted glycoproteins of adult Schistosoma bovis worms

AuthorsRamajo Hernández, Alicia ; Oleaga, Ana ; Ramajo Martín, Vicente ; Pérez Sánchez, Ricardo
KeywordsSchistosoma bovis
Glycans
Glycoproteins
Lewis antigens
Mass spectrometry
Issue Date15-Mar-2007
PublisherElsevier
CitationVeterinary Parasitology 144(1-2): 45-60 (2007)
AbstractSchistosoma bovis is a parasite of wild and domestic ruminants that is broadly distributed throughout many tropical and temperate regions of the old world. S. bovis causes severe health problems and significant economic losses in livestock, but in contrast to human schistosomes, S. bovis has been little investigated at a molecular level. Since schistosome glycans and glycoproteins can play important roles in the host–parasite interplay, the aims of the present work were: (i) to characterize the glycans expressed by adult S. bovis worms on their excreted/secreted (ES) and tegumental (TG) glycoproteins and (ii) to identify their carrier protein backbones by mass spectrometry. Using a panel of lectins and monoclonal and polyclonal anti-glycan antibodies, we observed: (i) the absence of sialic acid in S. bovis; (ii) the presence of complex-type N-glycans and LDN antennae on ES glycoproteins; (iii) the presence of glycans containing the Fucα1-2Galβ motif in many TG glycoproteins, and (iv) the presence of glycans containing the Fucα1-3GlcNAc motif on many ES and TG glycoproteins but, simultaneously, the absence of the F-LDN(-F) glycans from both the ES and TG glycoproteins. Interestingly, we also found the Lewis(X) and Lewis(Y) antigens co-expressed on several TG isoforms of ATP:guanidino kinase and glyceraldehyde-3-phosphate dehydrogenase. Finally, by ELISA we observed the presence of antibodies against Lewis(X), Lewis(Y) and F-LDN(-F) in the sera of sheep experimentally infected with S. bovis.
Description16 pages, 5 figures.-- PMID: 17055171 [PubMed].-- Available online Oct 18, 2006.
Publisher version (URL)http://dx.doi.org/10.1016/j.vetpar.2006.09.034
URIhttp://hdl.handle.net/10261/10328
DOI10.1016/j.vetpar.2006.09.034
ISSN0304-4017
Appears in Collections:(IRNASA) Artículos
Files in This Item:
File Description SizeFormat 
60292_Vetpar-D-06-903_final_manuscript.pdf3,23 MBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.