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Title

Haptens, bioconjugates, and antibodies for penthiopyrad immunosensing

AuthorsCeballos Alcantarilla, Eric ; Abad Fuentes, Antonio ; Aloisio, Vincenzo; Agulló, Consuelo; Abad Somovilla, Antonio; Mercader Badia, Josep Vicent
KeywordsPenthiopyrad
Fungicides
Immunochemical analysis
Antibodies
Issue Date26-Aug-2014
PublisherRoyal Society of Chemistry (UK)
CitationAnalyst 39: 5358-5361 (2014)
AbstractHaptens, bioconjugates, and antibodies for highly sensitive immunochemical analysis of the new-generation fungicide penthiopyrad are described. Two haptens with equivalent carboxylated linkers were prepared, and the purified active esters were efficiently coupled to proteins. The results revealed slightly different antibody-eliciting capacities for the two synthetic derivatives. All of the produced antibodies were specific for penthiopyrad, and showed affinity values in the nanomolar range.
Description4 pages, 1 table, 2 figures.
Publisher version (URL)http://dx.doi.org/10.1039/C4AN00828F
URIhttp://hdl.handle.net/10261/102973
DOI10.1039/c4an00828
Appears in Collections:(IATA) Artículos
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