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dc.contributor.authorViguera, Enrique-
dc.contributor.authorHernandez, Pablo-
dc.contributor.authorKrimer, Dora B.-
dc.contributor.authorLurz, Rudi-
dc.contributor.authorSchvartzman, Jorge Bernardo-
dc.date.accessioned2014-10-06T11:14:14Z-
dc.date.available2014-10-06T11:14:14Z-
dc.date.issued2000-01-
dc.identifier.citationNucleic Acids Research 2000,28 (2) 498–503es_ES
dc.identifier.issn0305-1048-
dc.identifier.urihttp://hdl.handle.net/10261/102950-
dc.description6 p.-4 fig.es_ES
dc.description.abstractBlockage of replication forks can have deleterious consequences for the cell as it may prompt premature termination of DNA replication. Moreover, the blocked replication intermediate (RI) could be particularly sensitive to recombination processes. We analysed the different populations of RIs generated in vivo in the bacterial plasmid pPI21 after pausing of replication forks at the inversely oriented ColE1 origin. To achieve this goal, a new method was developed based on two-dimensional agarose gel electrophoresis. This method allows the isolation of specific RIs, even when they were rather scarce, from the total DNA. Here we describe the occurrence of RI restriction fragments containing reversed forks. These Holliday-like structures have been postulated but never observed before.es_ES
dc.description.sponsorshipThis work was partially supported by grants 93/0161, 96/0470 and 99/0850 from the Spanish Fondo de Investigación Sanitaria, grant 08.6/0016/1997 from the Comunidad Autónoma de Madrid, and grant PM97-0138 from the Spanish Comisión Interministerial de Ciencia y Tecnología (CICYT).es_ES
dc.language.isoenges_ES
dc.publisherOxford University Presses_ES
dc.relation.isversionofPublisher's versiones_ES
dc.rightsopenAccesses_ES
dc.titleVisualisation of plasmid replication intermediates containing reversed forkses_ES
dc.typeartículoes_ES
dc.identifier.doi10.1093/nar/28.2.498-
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionhttp://dx.doi.org/10.1093/nar/28.2.498es_ES
dc.identifier.e-issn1362-4962-
dc.relation.csices_ES
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