English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/10287
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Exportar a otros formatos:


Comprehensive liquid chromatography–ion-spray tandem mass spectrometry method for the identification and quantification of eight hydroxylated brominated diphenyl ethers in environmental matrices

AuthorsMas García, Sílvia ; Jáuregui, Olga; Rubio, Fernando; Juan, Anna de; Tauler, Romà ; Lacorte Bruguera, Silvia
KeywordsHydroxylated brominated diphenyl ethers (OH-PBDEs)
Liquid chromatography-ion-spray tandem mass spectrometry
Multiple reaction monitoring
Quality parameters
Environmental samples
Issue Date18-May-2007
PublisherJohn Wiley & Sons
CitationJournal of Mass Spectrometry, 42(7): 890-899 (2007)
AbstractWe propose an instrumental method based on liquid chromatography coupled to negative ionspray ionization (ISP(−)) tandem mass spectrometry (MS/MS), for the simultaneous analysis of eight hydroxylated brominated diphenyl ethers (OH-PBDEs) in four different environmental matrices (soil, fish, sludge and particulate matter). The reversed-phase chromatographic separation was performed on a 50 mm Xbridge C18 column, and the compounds were well resolved with a gradient consisting of a ternary mixture of 5 mMammonium acetate, methanol and acetonitrile. Detection was performed in the multiple reaction monitoring (MRM) mode using the [M − H](−) ion as base peak. The fragmentation pathways of the OH-PBDEs varied according to the hydroxyl substitution in the benzene rings and produced characteristic MRM transitions needed for resolving isomeric compounds. The method is acceptable for quantification in the high picogram per gram dry weight (dw) level for allmatrices analyzed. Repeatability and reproducibility tested at 75 pg μl−1 were below 10% using internal standard quantification. The ISP(−) enhancement due to matrix effects was in the 76–132% range and the highest values corresponded to sludge samples. The use of the proposed method based on LC-ISP(−)-MS/MS opens a new way to directly determine OH-PBDEs without the need of derivatization.
Description10 pages, 4 tables, 2 figures.-- PMID: 17511022 [PubMed].
Publisher version (URL)http://dx.doi.org/10.1002/jms.1224
Appears in Collections:(IDAEA) Artículos
Files in This Item:
There are no files associated with this item.
Show full item record
Review this work

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.