English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/10283
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
DC FieldValueLanguage
dc.contributor.authorFernandes, Denise-
dc.contributor.authorBebianno, Maria João-
dc.contributor.authorPorte Visa, Cinta-
dc.date.accessioned2009-02-04T12:19:31Z-
dc.date.available2009-02-04T12:19:31Z-
dc.date.issued2007-09-18-
dc.identifier.citationAquatic Toxicology 85(4): 258-266 (2007)en_US
dc.identifier.issn0166-445x-
dc.identifier.urihttp://hdl.handle.net/10261/10283-
dc.description9 pages, 4 figures.-- PMID: 17977610 [PubMed].-- Printed version published on Dec 30, 2007.en_US
dc.description.abstractThe metabolism of 17α-hydroxyprogesterone (17P4) was investigated in different subcellular fractions isolated from male gonads of sea bass (Dicentrarchus labrax L). The existence of CYP17 (C17,20-lyase activity) and CYP11B (11β-hydroxylase) catalyzed reactions was demonstrated in the mitochondrial fraction, where 17P4 was converted to androstenedione (AD) and further metabolized to 11β-hydroxyandrostenedione (βAD). The synthesis of βAD predominated in early spermatogenic testis, indicating a role of βAD in testicular recrudescence. Additionally, the in vitro effect of model endocrine disrupting chemicals (i.e. nonylphenol (NP), p,p'-DDE, benzo[a]anthracene (BaA), tributyltin (TBT) and ketoconazole (KCZ)) on the mitochondrial metabolism of 17P4 was investigated. Among the tested compounds, 100 μM NP inhibited the activity of CYP17 (C17,20-lyase) whereas 100 μM KCZ inhibited both CYP17 and CYP11B. Both chemicals showed the potential to disrupt the reproductive cycle of fish living in polluted environments due to impairment of testicular steroid biosynthesis. These results suggest that mitochondrial metabolism of 17P4 may constitute a new sensitive probe for the assessment of endocrine disruption in fish.en_US
dc.description.sponsorshipThis study was supported by the Spanish Ministry of Science and Education under Project Ref. CGL2005-02846. Denise Fernandes acknowledges a PhD fellowship (SFRH/BD/6123/2001) from the Portuguese Fundaçao para a Ciência e Tecnologia (FCT) of the Ministry of Science and Technology of Portugal.en_US
dc.format.extent19968 bytes-
dc.format.mimetypeapplication/msword-
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.rightsclosedAccessen_US
dc.subjectSea bassen_US
dc.subjectMitochondriaen_US
dc.subject17α-Hydroxyprogesteroneen_US
dc.subjectNonylphenolen_US
dc.subjectC17,20-lyaseen_US
dc.subject11β-Hydroxylaseen_US
dc.titleMitochondrial metabolism of 17α-hydroxyprogesterone in male sea bass (Dicentrarchus labrax): A potential target for endocrine disruptorsen_US
dc.typeartículoen_US
dc.identifier.doi10.1016/j.aquatox.2007.09.010-
dc.description.peerreviewedPeer revieweden_US
dc.relation.publisherversionhttp://dx.doi.org/10.1016/j.aquatox.2007.09.010en_US
Appears in Collections:(IDAEA) Artículos
Files in This Item:
There are no files associated with this item.
Show simple item record
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.