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Title

Mitochondrial metabolism of 17α-hydroxyprogesterone in male sea bass (Dicentrarchus labrax): A potential target for endocrine disruptors

AuthorsFernandes, Denise; Bebianno, Maria João; Porte Visa, Cinta
KeywordsSea bass
Mitochondria
17α-Hydroxyprogesterone
Nonylphenol
C17,20-lyase
11β-Hydroxylase
Issue Date18-Sep-2007
PublisherElsevier
CitationAquatic Toxicology 85(4): 258-266 (2007)
AbstractThe metabolism of 17α-hydroxyprogesterone (17P4) was investigated in different subcellular fractions isolated from male gonads of sea bass (Dicentrarchus labrax L). The existence of CYP17 (C17,20-lyase activity) and CYP11B (11β-hydroxylase) catalyzed reactions was demonstrated in the mitochondrial fraction, where 17P4 was converted to androstenedione (AD) and further metabolized to 11β-hydroxyandrostenedione (βAD). The synthesis of βAD predominated in early spermatogenic testis, indicating a role of βAD in testicular recrudescence. Additionally, the in vitro effect of model endocrine disrupting chemicals (i.e. nonylphenol (NP), p,p'-DDE, benzo[a]anthracene (BaA), tributyltin (TBT) and ketoconazole (KCZ)) on the mitochondrial metabolism of 17P4 was investigated. Among the tested compounds, 100 μM NP inhibited the activity of CYP17 (C17,20-lyase) whereas 100 μM KCZ inhibited both CYP17 and CYP11B. Both chemicals showed the potential to disrupt the reproductive cycle of fish living in polluted environments due to impairment of testicular steroid biosynthesis. These results suggest that mitochondrial metabolism of 17P4 may constitute a new sensitive probe for the assessment of endocrine disruption in fish.
Description9 pages, 4 figures.-- PMID: 17977610 [PubMed].-- Printed version published on Dec 30, 2007.
Publisher version (URL)http://dx.doi.org/10.1016/j.aquatox.2007.09.010
URIhttp://hdl.handle.net/10261/10283
DOI10.1016/j.aquatox.2007.09.010
ISSN0166-445x
Appears in Collections:(IDAEA) Artículos
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