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Determination of the antimicrobial growth promoter moenomycin-A in chicken litter

AutorPérez Solsona, Sandra; McJury, Brenna E.; Eichhorn, Peter; Aga, Diana S.
Palabras claveMoenomycin
Liquid chromatography–electrospray ionization–mass spectrometry (LC–ESI–MS)
Chicken litter
Chicken feed
Fecha de publicación24-oct-2007
CitaciónJournal of Chromatography A 1175(2): 234-241 (2007)
ResumenThis study aimed to develop and optimize a method for the extraction and analysis of moenomycin antibiotics (a.k.a. flavomycin) in corn-based feed premix and in chicken litter. Moenomycin-A was isolated from chicken litter using pressurized liquid extraction followed by a solid-phase extraction (SPE) clean-up step. The highly lipophilic nature of moenomycin necessitated the use of the less hydrophobic sorbent, C4-based SPE cartridge, and a higher temperature elution solvent, methanol at 50ºC, in order to obtain satisfactory percent recoveries. After clean-up, the sample was analyzed by liquid chromatography–electrospray ionization–mass spectrometry (LC–ESI–MS). Various reversed-phase columns were examined, including C18, CN, perfluorinated C6, and a porous graphitic carbon. The set of conditions that gave the highest separation efficiency while still maintaining symmetrical peak shape was the C18 column using the H2O + 0.3% HCOOH and acetonitrile mobile phase.
Descripción8 pages, 6 figures, 2 tables.-- PMID: 17996876 [PubMed].-- Printed version published on Dec 21, 2007.
Versión del editorhttp://dx.doi.org/10.1016/j.chroma.2007.10.053
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