Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/102437
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | The CDK regulators Cdh1 and Sic1 promote efficient usage of DNA replication origins to prevent chromosomal instability at a chromosome arm |
Autor: | Ayuda-Milán, Pilar; Devesa, Fernando; Gomes, Fábia; Sequeira-Mendes, Joana CSIC ORCID; Ávila-Zarza, Carmelo; Gómez, María CSIC ORCID; Calzada, Arturo CSIC ORCID | Palabras clave: | DNA replication Genome instability Cell cycle regulation Cdh1 Sic1 |
Fecha de publicación: | 21-abr-2014 | Editor: | Oxford University Press | Citación: | Nucleic Acids Research 42(11): 7057-7068 (2014) | Resumen: | Robustness and completion of DNA replication rely on redundant DNA replication origins. Reduced efficiency of origin licensing is proposed to contribute to chromosome instability in CDK-deregulated cell cycles, a frequent alteration in oncogenesis. However, the mechanism by which this instability occurs is largely unknown. Current models suggest that limited origin numbers would reduce for kdensity favouring chromosome rearrangements, but experimental support in CDK-deregulated cells is lacking. We have investigated the pattern of origin firing efficiency in budding yeast cells lacking the CDK regulators Cdh1 and Sic1. We show that each regulator is required for efficient origin activity, and that both cooperate non-redundantly. Notably, origins are differentially sensitive to CDK deregulation. Origin sensitivity is independent on normal origin efficiency, firing timing or chromosomal location. Interestingly,at a chromosome arm, there is a shortage of origin firing involving active and dormant origins, and the extent of short age correlates with the severity of CDK deregulation and chromosome instability. We therefore propose that CDK deregulation in G1 phase compromises origin redundancy by decreasing the number of active and dormant origins, leading to origin short age and increased chromosome instability. | Versión del editor: | http://dx.doi.org/10.1093/nar/gku313 | URI: | http://hdl.handle.net/10261/102437 | DOI: | 10.1093/nar/gku313 | E-ISSN: | 1362-4962 |
Aparece en las colecciones: | (CNB) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
Nucl. Acids Res.-2014.pdf | 995,5 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
PubMed Central
Citations
7
checked on 14-abr-2024
SCOPUSTM
Citations
12
checked on 16-abr-2024
WEB OF SCIENCETM
Citations
11
checked on 25-feb-2024
Page view(s)
342
checked on 17-abr-2024
Download(s)
258
checked on 17-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
Artículos relacionados:
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.